Molecular Biology 174 Centrifugal ultrafiltration device for dilute protein solutions. Insert tube with ultrafiltration membrane. For the concentration and purification of proteins, antibodies and nucleic acids, desalting and diafiltration, removal of unincorporated primers, linkers and markers. hVery high reproducibility hNumerous cut-off points available hVery high sample recovery (›90%) due to reverse centrifugation method hFor PCR products purification, use 30 kDa units Amicon Ultra 2 ml Max. initial volume 2 ml 0.5 ml Final volume 15 - 70 µl 15 - 20 µl Max centrifugal force 4000 g (fixed rotor) 14000 g 7500 g (tilting rotor) Tube format - Microtube 1.5 ml Amicon Ultra 0.5 ml 0.5 ml 2 ml Merck Millipore ULTRAFiltration / concentration With very low protein adsorption, it can concentrate and desalinate very dilute solutions. Its ultra-fine microstructure allows very high retentions with extremely low specific adsorption of proteins, DNA or other macromolecules. This membrane is available on the Amicon Ultra and Centricon Plus70 range. Ultracel ultrafiltration membrane (made of regenerated cellulose) With low protein adsorption and low extractables, it can be used to clarify samples, recover DNA from agarose gels and retain chromatography resins. Durapore membranes allow the passage of soluble proteins or nucleic acids and retain cell fragments, whole cells or particles. The Durapore membrane is extremely hydrophilic and provides the lowest adsorption to proteins or other biomolecules of any microporous membrane on the market. The Durapore membrane is used in the Ultrafree-MC and CL. Durapore microporous membrane (PVDF) Membrane Ultrafiltration Proteins, DNA, viruses Salts, sugars, amino acids, oligonucleotides Microporous Cells, precipitates, resins, beads, particles Salts, proteins, nucleic acids, viruses Retention Passage Sample type Dilute, extremely pure sample requiring low adsorption Ultracel Regenerated Cellulose 3000 Da, 10000 Da, 30000 Da, 50000 Da, 100000 Da Cells, precipitate, bead, resin requiring the passage of molecules Durapore hydrophilic PVDF 0.1 µm, 0.2 µm, 0.45 µm, soluble such as proteins, nucleic acids or salts 0.65 µm, 5 µm Membrane Material Cut-off point See Amicon™ Ultra, Amicon™ Pro next page Amicon Ultra 0.5 ml Amicon Ultra 2 ml Threshold of kDa cut-off Cat.No. 8 units € 8 units Cat.No. 24 units € 24 units Cat.No 96 units € 96 units Cat.No. 500 units € 500 units Cat.No. 24 units € 24 units 3 051823 N/A - 051824 N/A - 051826 N/A - 051831 N/A - 053716 N/A - 10 051828 N/A - 051829 N/A - 051830 N/A - 051827 N/A - 053717 N/A - 30 051850 N/A - 051851 N/A - 051852 N/A - 051853 N/A - 053718 N/A - 50 051854 N/A - 051855 N/A - 051856 N/A - 051857 N/A - 053719 N/A - 100** 051858 N/A - 051859 N/A - 051860 N/A - 051861 N/A - 053720 N/A - ** Max. centri force: 5000 g for proteins / 2000 g for nucleic acids Ultrafiltration / Amicon Merck Millipore's Amicon family of products offers you the widest selection of ultrafiltration products for protein processing and molecular biology applications. Centrifugal filtration allows biological samples to be concentrated, desalted, diafiltered and clarified quickly and reproducibly. Centrifugal filtration also reduces processing times, increases sample recovery efficiency, allows higher concentration factors to be achieved and further desalination of samples. The choice of a centrifuge unit will depend on several factors: the starting concentration of the sample; the starting volume; the desired concentration factor; the user's centrifuge equipment. While some units favour high sample recovery efficiencies, others will allow for high ultrafiltration rates. Amicon Ultra 0.5 and 2 ml Enter the Cat. No. on www.dulis.nl to see the Investigative Genetics guide. WEB
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