Molecular Biology 176 For DNA extraction from agarose gels Tube for the recovery of 100 to 10 000 bp DNA fragments from agarose gels. The kit includes 50 DNA extraction microtubes and 500 ml of modified 50 x TAE buffer (0.1 mM EDTA instead of 1 mM EDTA). hSimple: just place a piece of gel in the nebuliser of the unit hFast: 10 minute centrifugation hFully functional DNA hNo further steps required hSingle use, avoids cross-contamination hIdeal for use with radioactive samples h2 ml microtube format: 10.6 x 45 mm hfiltration area: 0.2 cm2 hCentrifugal force: 5000 g Exploded view of an Ultrafree-DA: 1 - Gel 2 - Gel nebulizer 3 - Filter insert 4 - Microcentrifuge tube 5 - DNA Cat. No. 051343 LSKGEL050 Gel Extraction KiNC Assembly 50 N/A - Cat. No. Millipore Description €/ box Units/ box Merck Millipore ULTRAFiltration / concentration Max. Volume 500 µl 2 ml Centrifugal force 12000 g 5000 g Filtration area (cm2) 0.2 0.8 Centrifuge tube Microtube 2 ml Tube 15 ml Dead volume ‹ 5 µl ‹ 10 µl ø x W (mm) 10.6 x 45 16.3 x 77 Microfiltration on 2 ml microtubes. Durapore (PVDF) membrane. For the removal of particles and precipitates from aqueous samples, ideal for use with nucleic acid and protein solutions. Ultrafree-MC Ultrafree-CL Pores size (µm) Colour 0.1 Orange Cat. No. 051014 051027 Units/box 25 25 €/box N/A - N/A - 0.22 Yellow Cat. No. 051015 051028 Units/box 25 25 €/box N/A - N/A - 0.45 Red Cat. No. 051016 051029 Units/box 25 25 €/box N/A - N/A - 0.65 Violet Cat. No. 051017 051030 Units/box 25 25 €/box N/A - N/A - 5 Dark Green Cat. No. 051018 051031 Units/box 100 25 €/box N/A - N/A - Ultrafree-MC Ultrafree-CL Centrifugal filtration units with Ultracel PL membrane (high recovery). hHigh recovery rate (› 90 %) hFor use in a 250 ml tube rotor with movable cups h Applications hConcentration, purification, desalting of proteins hConcentration and desalting of chromatographic eluates hConcentration of monoclonal antibodies, viruses hClarification of cell homogenates hPurification of cell lysates Initial maximum volume (ml) 70 Final concentrate volume (µl) 350 Centrifugal force 3500 g Filtration area (cm2) 19 ø x W (mm) 60 x 121 Tube Format 250 ml Centricon Plus-70 Cut-off point Da Cat.No. Units/ box €/ box 10000 051555 8 N/A - 30000 051556 8 N/A - 100000 051557 8 N/A - Ultrafree MC - Ultrafree CL filtration tubes Centricon Plus-70 Centrifuge Filters Gel Extraction DNA Assembly Kit
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