Molecular Biology 196 Blotting, hybridization and revelation Membranes and blotting papers for nucleic acids hVery high positive charge, low background noise hHigher nylon density than with other membranes increases sample fixation capacity hExcellent symmetry: always remains flat hNytran Super Charge membrane binds nine times more molecules than a conventional nylon membrane hConsistent membrane morphology: good uniformity of nylon distribution and therefore of loads Cat.No. Nytran SuperCharge €/ box Units/ box Dim. (mm) Type 034779 0.45 µm 110 x 140 Sheets 10 N/A - 034798 0.45 µm 200 x 3 m Rolls 1 N/A - 034799 0.45 µm 300 x 3 m Rolls 1 N/A - Nytran Super Charge (SPC) Nylon Transfer Membrane h Hybond-N hResistant hFor nucleic acid blotting hFixing capacity 600 µg/cm2 hFor radioactive applications hUV light fixing h Hybond-N+ hFor nucleic acid blotting hFixing capacity 600 µg/cm2 hFor detection by radioactivity, chemiluminescence, chemifluorescence h Hybond-NX hFor nucleic acid blotting hFixing capacity 600 µg/cm2 hDeveloped specifically for use with low volumes of hybridization buffer and high flow applications hGenerates little background noise h Hybond-XL hFor nucleic acid blotting hFixing capacity 600 µg/cm2 hEffective fixation of small fragments hLoaded nylon for optimised signal-to-noise ratio when working with radioactively labelled samples Cat. No. Dimensions Pore size Membrane material € the roll Hybond-NX in neutral nylon, for more demanding applications Hybond-XL in nylon optimised for radio-labelled probes RPN303N 30 cm x 3 m 0.45 µm Nylon N/A - RPN203N 20 cm x 3 m 0.45 µm Nylon N/A - RPN303B 30 cm x 3 m 0.45 µm Positively charged nylon N/A - RPN203B 20 cm x 3 m 0.45 µm Positively charged nylon N/A - RPN303T 30 cm x 3 m 0.45 µm Nylon N/A - RPN203T 20 cm x 3 m 0.45 µm Nylon N/A - RPN303S 30 cm x 3 m 0.45 µm Positively charged nylon N/A - RPN203S 20 cm x 3 m 0.45 µm Positively charged nylon N/A - Hybond-N in neutral nylon Hybond-N + in positively charged nylon, versatile Other sizes available: please contact us. Amersham™ HYBOND blotting membranes
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