Molecular Biology 198 Blotting, hybridization and revelation Membrane and blotting paper for proteins hReady-to-use solution for blotting applications hContains two sheets of 3MM CHR paper of appropriate size for each membrane sheet hThe membrane is to be placed on the anode side of the gel hThe 3MM CHR paper sheets cover each side of the entire hThe gel is ready for transfer Older range New range Use "Amersham Hybond P Whatman Westran CS" Amersham Hybond P Chemiluminescence, compatible with fast bleaching solvents Whatman Westran S Amersham Hybond SEQ Sequencing and solid phase analysis Amersham Hybond LFP Amersham Hybond LFP Fluorescence (low membrane autofluorescence) Packaging Amersham Hybond P Amersham Hybond SEQ Amersham Hybond LFP Pore size Size (mm) Units/ box Cat. No. €/ box Cat. No. €/ box Cat. No. €/ box 0.2 µm 80 x 90 25 10600101 N/A - - - 10600102 N/A - 100 x 100 10 10600089 N/A - - - - - 200 x 200 10 10600058 N/A - - - 10600060 N/A - 25 10600057 N/A - - - - - 260 x 600 5 10600039 N/A - 10600030 N/A - 10600040 N/A - 260 x 4 m Roll 10600021 N/A - - - 10600022 N/A - 0.45 µm 80 x 90 25 10600100 N/A - - - - - 100 x 100 10 10600087 N/A - - - - - 25 10600086 N/A - - - - - 140 x 160 25 10600069 N/A - - - - - 300 x 600 5 10600038 N/A - - - - - 150 x 4 m Roll 10600029 N/A - - - - - 300 x 4 m Roll 10600023 N/A - - - - - Sandwich 0.2 µm 80 x 90 10 10600122 N/A - - - 10600123 N/A - Sandwich 0.45 µm 80 x 90 10 10600121 N/A - - - - - 140 x 160 10 10600110 N/A - - - - - Blotting paper Gel (not included) Membrane Blotting paper Amersham Hybond PVDF transfer membranes h100% pure nitrocellulose membrane 0.1 µm for ‹ 7 kD molecules hHigh fixation, low background noise hHigh retention of small proteins: - the 0.2 µm membrane retains samples smaller than 20 KD - the 0.45 µm membrane is designed for molecules larger than 20 KD hExcellent protein stability on Protran (› 5 years) hNo pre-wetting with methanol: before transfer, a simple wetting with water is sufficient hExcellent stability of the membrane: allows many manipulations Cat.No. Protran NC model €/ box Units/ box Dim. (mm) Type 034714 BA 83 0.2 µm 82 Discs 50 N/A - 034735 BA 85 0.45 µm 25 Discs 100 N/A - 034738 BA 85 0.45 µm 25 Discs 1000 N/A - 034701 BA 85 0.45 µm 132 Discs 25 N/A - 034736 BA 85 0.45 µm 132 Discs 50 N/A - 034737 BA 85 0.45 µm 137 Discs 50 N/A - Protran NC nitrocellulose transfer membrane
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