Molecular Biology 199 All prices are indicative and exclude VAT. Visit www.dulis.nl Immobilon-FL (PVDF) transfer membranes Optimised for applications with fluorescence detection hSame fixation characteristics as Immobilon-P (PVDF, 0.45 µm) hVery low background noise in fluorescence detection in western blotting hCompatible with the most commonly used fluorescence reagents hCompatible with non-fluorescent detection reagents Immobilon Sandwiches (PVDF) Transfer Membranes hPre-cut hSupplied with buffer, separators and absorbent sheets hCompatible with pre-cast gels Immobilon-NC transfer membranes (cellulose esters) Immobilon-NC HAHY membranes made of cellulose esters with surfactants, which improves their wettability and facilitates their handling during the transfer process. Immobilon™-Ny+ transfer membranes For greater sensitivity during re-hybridisation. Immobilon-Ny+ membranes optimised for reliable and reproducible transfer, immobilisation, hybridisation and rehybridisation. ImmobilonTM Transfer membranes Immobilon-P (PVDF) transfer membranes Immobilon-E (PVDF) transfer membranes Made of polyvinylidene fluoride (PVDF)hydrophobic, optimised for binding proteins transferred from different types of gels. hIdeal substrate for immunodetection hUsing the rapid protocol developed by Merck Millipore, time savings of 2 hours: elimination of the blocking step in a western blot, reduction of the number and duration of washes, without compromising specificity or sensitivity Immobilon-PSQ (PVDF) transfer membranes Made of polyvinylidene fluoride (PVDF) for the transfer of low molecular weight proteins. Immobilon-PSQ membranes: for blotting of ‹ 20 kD proteins hHigh yields in protein sequencing hNo surfactant hDo not require a methanol membrane activation step hBetter protein retention and durability than nitrocellulose membranes hCan be moistened directly with transfer buffer hCompatible detection methods: chemiluminescence, chromogenic and radioactivity hCompatible with Coomassie Blue, Ponceau-S Red, Sypro Ruby and colloidal gold dyes hMembranes Cat. No. 044435 and 044436 are supplied with blotting paper sheets Cat.No. Merck Millipore Cat. No. Porosity (µm) Presen- tation Dim. (mm) Units/ box €/ box 044430 IEVH00005 0.45 Roll 265 x 1875 1 N/A - 044431 IEVH07850 0.45 Sheets 70 x 84 50 N/A - 044432 IEVH08100 0.45 Sheets 80 x 100 10 N/A - 044433 IEVH09120 0.45 Sheets 90 x 120 10 N/A - 044434 IEVH10100 0.45 Sheets 100 x 100 10 N/A - 044435 IESN07852 0.45 Sheets 70 x 84 20 N/A - 044436 IESN08132 0.45 Sheets 85 x 135 20 N/A - 044437 IEVH07804 0.45 Sheets 70 x 84 4 N/A - Cat.No. Merck Millipore Cat. No. Porosity (µm) Presen- tation Dim. (mm) Units/ box €/ box 051587 IPFL10100 0.45 Sheets 100 x 100 10 N/A - 051588 IPFL20200 0.45 Sheets 200 x 200 10 N/A - 051589 IPFL00010 0.45 Roll 265 x 3750 1 N/A - Cat.No. Merck Millipore Cat. No. Porosity (µm) Presen- tation Dim. (mm) Units/ box €/ box 051590 IPSN07852 0.45 Sheets 70 x 84 20 N/A - 051591 IPSN08132 0.45 Sheets 85 x 135 20 N/A - Cat.No. Merck Millipore Cat. No. Porosity (µm) Presen- tation Dim. (mm) Units/ box €/ box 044067 INYC00010 0.45 Roll 300 x 3300 1 N/A - Cat.No. Merck Millipore Cat. No. Porosity (µm) Presen- tation Dim. (mm) Units/ box €/ box 044055 HATC00010 0.45 Roll 330 x 3000 1 N/A - Cat.No. Merck Millipore Cat. No. Porosity (µm) Presen- tation Dim. (mm) Units/ box €/ box 044088 ISEQ00010 0.2 Roll 265 x 3750 1 N/A - 044089 ISEQ15150 0.2 Sheets 150 x 150 10 N/A - 044090 ISEQ101000 0.2 Sheets 100 x 100 10 N/A - 044091 ISEQ20200 0.2 Sheets 200 x 200 10 N/A - 044092 ISEQ26260 0.2 Sheets 260 x 260 10 N/A - Blotting, hybridization and revelation Membrane and blotting paper for proteins Cat.No. Merck Millipore Cat. No. Porosity (µm) Presen- tation Dim. (mm) Units/ box €/ box 044081 IPVH07850 0.45 Sheets 70 x 84 50 N/A - 044082 IPVH304F0 0.45 Sheets 260 x 260 10 N/A - 044083 IPVH09120 0.45 Sheets 90 x 120 10 N/A - 044084 IPVH10100 0.45 Sheets 100 x 100 10 N/A - 044085 IPVH20200 0.45 Sheets 200 x 200 10 N/A - 044086 IPVH15150 0.45 Sheets 150 x 150 10 N/A - 044087 IPVH00010 0.45 Roll 265 x 3750 1 N/A -
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