Molecular Biology 201 All prices are indicative and exclude VAT. Visit www.dulis.nl Blotting, hybridization and revelation Detection and blocking Cat. No. Description € 053777 Luminata Forte Elisa HRP Substrate 100 ml N/A - 053778 Luminata Crescendo Elisa HRP Substrate 100 ml N/A - 053779 Luminata Crescendo Elisa HRP Substrate 200 ml N/A - Luminata Elisa HRP Amersham ECL Select™ Amersham ECL Start™ Amersham ECL™ Amersham ECL Prime™ Contains: hECL Plex Goat - mouse IgG-Cy3, 150 µg hECL Plex Goat - rabbit IgG-Cy5, 150 µg hHybond ECL 10 x 10 cm (cdt 10) or Hybond-LFP 20 x 20 cm (cdt 3) hMarqueurs ECL Plex Rainbow 120 µl Amersham ECL Plex™: for quantitative detection Use of 2 fluorescent markers allowing the simultaneous detection of 2 proteins on western blots. Amersham ECL™ Amersham ECL Start™ Amersham ECL Prime™ Amersham ECL Select™ Amersham ECL Plex™ Kit features Routine Western blotting Confirmatory Western blotting for high to moderate sensitivity proteins Quantitative and confirmatory analysis High to low sensitivity proteins Quantitative and confirmatory analysis Medium to very low protein sensitivity Quantitative analysis Sensitivity (model system) ~10 pg N/C ~1 pg ~5 ng ~1.2 pg Primary antibody (model system) 1:100 - 1:5000 1:500 - 1:3000 1:1000 - 1:50000 1:5000 - 1:30000 1:100 - 1:5000 Secondary antibody (model system) 1:1000 - 1:15000 1:5000 - 1:50000 1:50000 - 1:250000 1:100000 - 1:300000 1:1250 - 1:4000 Emission duration 1 to 2 hrs 3 hrs 3 hrs 2 hrs › 3 months Reprobing with 2 antibodies Yes Yes Yes Yes Not required Recommended membrane Amersham Hybond P Amersham Protran Premium Amersham Hybond P Amersham Protran Amersham Hybond P Amersham Protran Premium Amersham Hybond Amersham Protran Premium Amersham Hybond LFP Amersham Protran Premium Recommended detection method Hyperfilm ECL and imageQuant imagers Amersham Hyperfilm and CCD camera detection range Hyperfilm ECL and imagers imageQuant Hyperfilm ECL and imagers imageQuant Fluorescence scanner and ImageQuant imagers hUses CyDye™ technology hMultiplex analysis of proteins Cat. No. Description € RPN3243 ECL Start for western blot 200 ml N/A - RPN3244 ECL Start for western blot 400 ml x 2 N/A - Cat. No. Description € RPN2108 ECL western blotting system for 1000 cm2 of membrane: anti-mouse HRP conjugate 100 µl, anti-Rabbit conjugate HRP 100 µl, detection reagents 1 and 2, blocking agent4 N/A - RPN2109 ECLWestern Blotting Detection Reagents for 1000 cm2 of membrane4 N/A - RPN2209 ECLWestern Blotting Detection Reagents for 2000 cm2 of membrane5 N/A - RPN2106 ECLWestern Blotting Detection Reagents for 4000 cm2 of membrane6 N/A - 4 contains 2 flasks of 62.5 ml 5 contains 2 flasks of 125 ml 6 contains 2 flasks of 250 ml Cat. No. Description € RPN2232 ECL PrimeWestern Blotting Reagent for 1000 cm2 of membrane1 N/A - RPN2236 ECL PrimeWestern Blotting Reagent for 3000 cm2 of membrane2 N/A - 1 contains 2 flasks of 50 ml 2 contains 2 flasks of 150 ml Cat. No. Description € RPN999 ECL Plex: CY3,CY5, RainbowMarker and HYBOND-LFP 20 x 20 cm N/A - Cat. No. Description € RPN2235 ECL Select Western blotting Reagent for 1000 cm2 of membrane3 N/A - 3 contains 1 x 50ml luminol flask and 1 x 50 ml peroxyde solution flask Protein labelling and detection Amersham ECL™, ECL Start™, ECL Advance™, ECL Select™, ECL Prime, hHRP reagent for chemoluminescent detection in ELISA format hRequires a suitable plate reader hHigh sensitivity to detect up to 0.1 pg of protein hWide dynamic range allowing a large number of detection thresholds hFlexible use, with two types of substrates, Luminata crescendo and Forte
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