MICROBIOLOGY Media Microbiology Reagents - Chemicals 579 All prices are indicative and exclude VAT. Visit www.dulis.nl Culture Media Dehydrated base Dehydrated base Ready-to-use media Ready-to-use media MRS deMan,Rogosa, Sharpe (Agar) MRS deMan,Rogosa, Sharpe pH 5.7 (Agar) For lactobacillus culture. The pH 5.4 medium allows the enumeration of Lactobacillus bulgaricus in yoghurt. hStorage: 2 - 8 °C (for dehydrated base and for ready-to-use media) hIncubation: 35 °C for 3 days or 30 °C for 5 days in a CO2enriched atmosphere The pH 5.7 medium allows the enumeration of mesophilic lactic acid bacteria hStorage: 2 - 8 °C (for dehydrated base and for ready-to-use media) hIncubation: 35 °C for 3 days or 30 °C for 5 days in a CO2enriched atmosphere Cat. No. Description Pack. € 777316 Dehydrated medium MRS agar - Condalab 500 g N/A - Cat. No. Description Pack. € 777858 Dehydrated medium MRS agar low pH ISO - Condalab 500 g N/A - Cat. No. Description Pack. € 693372 100 ml bottle of MRS agar pH 5.7 ClearLine® 10 N/A - Cat. No. Description Pack. € 693449 200 ml bottle of MRS agar - ClearLine® 6 N/A - MRVP (Methyl Red - Voges Proskauer) Clarks and Lubs Medium MSRV Modified Semisolid Rappaport Vassiliadis (Broth) For the differentiation of Escherichia coli from Entobacteria hStorage: 2 - 25 °C hIncubation: - For the methyl red test: 35 ± 2 °C for 3 to 5 days (Tube to be incubated before testing) - For the Voges Proskauer test: 35 ± 2 °C For the detection of mobile salmonella species according to ISO 6579 hStorage: 2 - 25 °C hIncubation: 41.5 °C for 24 h Cat. No. Description Pack. € 777320 Dehydrated medium MR-VP from Clark and Lubs - Condalab 500 g N/A - Cat. No. Description Pack. € 3390062.5 ml bottle of alpha naphthol + 2.5 ml bottle of potassium hydroxide for the VP test 10 + 10 N/A - Reagent required h5 drops of a 0.4% methyl red solution for the MR test Modified Rappaport Vassiliadis broth Cat. No. Description Pack. € 777319 Dehydrated medium MSRV medium - Condalab 500 g N/A - Mueller Hinton (Agar) Reference medium for antibiotic and sulphonamide susceptibility testing of germs. hStorage: 2 - 25 °C (for dehydrated base) and 2 - 8 °C (for ready-to-use media) hIncubation: 35 ± 2 °C for 24 h Dehydrated base Ready-to-use media Cat. No. Description Pack. € 777321 Dehydrated medium Mueller Hinton agar - Condalab 500 g N/A - Cat. No. Description Pack. € 693375 100 ml bottle of Mueller Hinton agar - ClearLine® 10 N/A - Supplement hAdd 20 ml/litre of iodide solution and 10 ml/litre of 0.1% bright green solution. To be used the same day Mueller Kauffman tetrathionate (Broth) For the selective enrichment of salmonella in food. hStorage: 2 - 25 °C hIncubation: 42 - 43 °C for 6 - 24 h Cat. No. Description Pack. € 777325 Dehydrated medium Mueller Kauffman Tetrathionate Broth - Condalab 500 g N/A - The neutralizer mixture is added to the pre-enrichment medium whenever the presence of disinfectant solution is known or suspected. As a general rule, this mixture should be used at a rate of 10% of the final volume of the pre-enrichment medium. Neutralizer hStorage 15 - 25 °C in the dark Formula in g/l Disodium phosphate 100.8 L-Histidine HCL 1 Sodium thiosulphate 7.8 Tween 80 30 Lecithin 3 Distilled water 857.4 Cat. No. Description Units/ box €/ box 693422 ClearLine® Neutralizer - Tube 25 ml 100 N/A - Universal neutralizer The neutralizer mixture is added to the pre-enrichment medium whenever the presence of disinfectant solution is known or suspected. As a general rule, this mixture should be used at a rate of 10% of the final volume of the pre-enrichment medium. Cat. No. Description Units/ box €/ box 693329 Universal neutralizer ClearLine® - 100 ml bottle 10 N/A - hStorage 15 - 25 °C in the dark Formula in g/l Disodium phosphate 75.45 Tween 20 20 Sodium thiosulphate 7.8 Tween 80 30 Lecithin 3 Distilled water 950 L-Histidine HCL 1
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