Table of Contents 565 566 567 568 569 570 571 572 573 574 575 576 577 578 579 580 581 582 583 584 585 586 587 588 589 590 591 592 593 594 595 596 597 598 599 600 601 602 603 604 605 606 607 608 609 IV

Media, microbiology and reagents

een uitzonderlijk avontuur DULIS bv - Nederland Marten Meesweg 25-G - 3068 AV Rotterdam www.dulis.nl I + 31 10-899 13 61 I info@dulis.nl

MEDIA, MICROBIOLOGY AND REAGENTS • Microbiology 565 à 577 • Media and products 578 to 589 • Broths, peptone water and agars, other media and ingredients 590 to 593 • Histology, dyes, decolorants, paraffin, reagents 594 and 595 • Chemicals 596 to 608 An extraordinary adventure

MICROBIOLOGY Media Microbiology Reagents - Chemicals 565 All prices are indicative and exclude VAT. Visit www.dulis.nl Microbiology Culture media (dehydrated and PAE) Founded in 1960, Condalab has become the leading Spanish producer of dehydrated culture media for microbiology and molecular biology. Through quality design and manufacture and sound technical expertise, Condalab now has an international presence in the laboratory. Strict controls during the production process ensure that the final product is of the highest quality and meets your performance requirements. Condalab supplies key ingredients for use in research, microbiology and quality control laboratories, such as agar, peptone and agarose, among other products. DULIS offers a wide range of agaroses as well as media and products for bacteriology, cloning and expression in molecular biology, in compliance with the European Pharmacopoeia (Eu. Pharm.), FDA, APHA, USP, AOAC and CeNAN, European Community (EC) stamped and ISO 9001:2000 certified. "Our mission is to make a major contribution to the life sciences through the design, production and delivery of products and services of the highest quality. Condalab's efforts are focused on improving efficiency, responsiveness and competitiveness in order to achieve our primary goal of providing world-class customer satisfaction. »

MICROBIOLOGY Media Microbiology Reagents - Chemicals 566 Microbiology Acetate differential agar Agar YPD Actinomycede isolation agar glycerol Anaerobic agar Bacillus cereus (selective agar) American bacteriological agar European bacteriological agar Baird-Parker Nitrogen base for amino acid-free yeast Industrial agar Pharmaceutical agar Purified agar Agar for plant propagation See Acetate differential agar See YPD agar See actinomycete isolation agar See Anaerobic agar See mossel (Bacillus cereus agar) Gelling agent for culture media. hStorage: 2 - 25 °C hGel strength: 600 - 850 g/cm 2 Gelling agent for culture media. hStorage: 2 - 25 °C hGel strength: 600 - 1100 g/cm 2 Recommended for the detection and enumeration of coagulase-positive staphylococci, including Stapylococcus aureus in food. hStorage: 2 - 25 °C (for dehydrated media) and 2 - 8 °C (for ready-to-use media) hIncubation: 35 ± 2 °C for 24 - 48 h hIncubation for ISO 11133: 37 ± 1 °C for 24 ± 2 h then 24 ± 2 h more For the classification of yeasts according to their amino acid and carbohydrate requirements. hStorage: 2 - 25 °C hIncubation: 25 - 30 °C for 2 - 5 days hStorage 8 - 14 °C h Composition: hStorage: 8 - 14 °C h Composition: Gelling agent for culture media. hStorage: 2 - 25 °C hGel strength: ‹ 850 g/cm 2 Gelling agent for culture media. hStorage: 2 - 25 °C hGel strength: 750 - 1000 g/cm 2 Gelling agent for culture media. hStorage: 2 - 25 °C hGel strength: 700 - 1200 g/cm 2 Gelling agent for culture media. hStorage: 2 - 25 °C hGel strength: › 900 g/cm 2 Cat. No. Description Pack. € 777475 American bacteriological agar - Condalab 500 g N/A - Cat. No. Description Pack. € 777476 European bacteriological agar Condalab 500 g N/A - 777525 Condalab European bacteriological agar 5 kg N/A - Cat. No. Description Pack. € 693396 ClearLine® Baird-Parker Agar - Flask 90 ml 10 N/A - 693350 ClearLine® Baird-Parker Agar - Flask 100 ml 10 N/A - Cat. No. Description Pack. € 777184 Baird-Parker agar dehydrated medium - Condalab 500 g N/A - Cat. No. Description Pack. € 777477 Condalab Industrial Agar 500 g N/A - Potassium tellurite 2.1 g Sodium chloride 4.25 g Egg yolk 200 ml Distilled water 800 ml Potassium tellurite 2.5 mg Trypsin 2.5 mg Rabbit plasma 2.5 ml Bovine fibrogen 0.38 g Cat. No. Description Pack. € 777523 RPF Supplement - Condalab 10 bottles N/A - Cat. No. Description Pack. € 777800 Egg yolk 100 ml bottle - Condalab 1 N/A - Cat. No. Description Pack. € 777479 Condalab Pharmaceutical Agar 500 g N/A - Cat. No. Description Pack. € 777480 Condalab Purified agar 500 g N/A - Cat. No. Description Pack. € 777508 Dehydrated nitrogen-based medium for amino acid-free yeast - Condalab 500 g N/A - Cat. No. Description Pack. € 777478 Agar for plant propagation - Condalab 500 g N/A - Agar LB (Lennox) Agar Luria See LB agar See Luria agar Dehydrated bases Ready-to-use media Supplement: egg yolk with tellurite (ISO 6888-1) Supplement: RPF (ISO 6888-2)

MICROBIOLOGY Media Microbiology Reagents - Chemicals 567 All prices are indicative and exclude VAT. Visit www.dulis.nl Microbiology Blood agar Eugon LT100 broth EE Broth Broth for yeast and mould Heart and brain broth Buffered sLB broth CCA (Chromogenic coliform agar) B.E.A - Bile esculin azide (agar) ISO 7899-2 Terrific broth Bryant Burkey with resazurin (broth) Campylobacter (agar) - ISO 10272 / ISO 11133 B.E.A - Bile esculin azide (agar) ISO 10273 B.L.B.V.B. - Milky Broth, Bright Green 2 % See Blood agar See eugon LT100 See mossel Broth See Yeast Mold broth See heart brain broth See sLB broth See E coli - coliform For the selective isolation and identification of enterococci by the membrane filtration method. hStorage 2 - 25 °C (dehydrated base and ready-to-use medium) hIncubation: 36 ± 2° C for 44 ± 4 h Used with glycerol for the culture of recombinant strains of Escherichia coli. For Molecular biology. hStorage: 2 - 25 °C hIncubation: 30 °C ± 2 °C for 18 - 24 h hStorage: 2 - 25 °C hIncubation: 37 ± 2 °C for 7 days For the isolation of Campylobacter jejuni, Campylobacter coli and Campylobacter laridis. hStorage: 8 - 25 °C hIncubation: 41.5 °C in a microaerobic atmosphere during 44 ± 4 h For the isolation and identification of enterococci and the study of esculin fermentation by Yersinia. hStorage: 2 - 25 °C hIncubation: 35 ± 2 °C for 18 - 24 h hFor the detection of Yersinia enterocolitica according to ISO 10273: 30 °C for 24 h hAddition of 10 ml of sodium lactate 50% hISO 10272 hTo be reconstituted in 5 ml sterile distilled water Cat. No. Description Pack. € 777186 Dehydrated medium for B.C.P. lactose agar - Condalab 500 g N/A - Cat. No. Description Pack. € 693351 100 ml bottle for Bromocresol Purple Lactose Agar - ClearLine® 10 N/A - Cat. No. Description Pack. € 777505 Dehydrated medium Terrific broth - Condalab 500 g N/A - Cat. No. Description Pack. € 777207 Bryant Burkey dehydrated broth medium with resazurin - Condalab 500 g N/A - Cat. No. Description Pack. € 777213 Dehydrated medium for Campylobacter agar - Condalab 500 g N/A - Cat. No. Description Pack. € 777776 Supplement for Campylobacter agar - Condalab 10 vials for 500 ml N/A - Cat. No. Description € 143307.1211-ITW Sodium Lactate 50% - 1 litre bottle N/A - Cat. No. Description Pack. € 777191 Dehydrated medium for B.E.A. agar (bile esculine azide agar) - ISO 7899-2 - Condalab 500 g N/A - Cat. No. Description Pack. € 693352 100 ml bottle of B.E.A. agar (bile esculin azide agar) - ISO 7899-2 - ClearLine® 10 N/A - Cat. No. Description Pack. € 777189 Dehydrated medium for B.E.A. agar (bile esculin azide agar) - ISO 10273 - Condalab 500 g N/A - Cat. No. Description Pack. € 777201 Dehydrated medium broth, bright green 2% - Condalab 500 g N/A - Dehydrated base Ready-to-use media Dehydrated medium Ready to use Supplement Supplement B.C.P. -Bromocresol Purple Lactose Agar - ISO 21528 Differential medium to guide the identification of lactose-fermenting and non-lactose-fermenting Enterobacteriaceae. hStorage: 2 - 25 °C (Dehydrated medium and ready-to-use medium) hIncubation: 35 ± 2 °C for 18 to 24 h For the detection of coliforms in water and food hStorage 2 - 8 °C hIncubation: 30 - 37 °C for 24 - 48 h

MICROBIOLOGY Media Microbiology Reagents - Chemicals 568 For the isolation and presumptive identification of Pseudomonas aeruginosa. Cetrimide is a quaternary ammonium that inhibits the growth of most other bacterial species. Pseudomonas aeruginosa stains this medium blue-green by producing pyocyanin. hStorage: 2 - 25 °C (dehydrated base and ready-to-use medium) hIncubation: 35 °C ± 2 °C for 18 - 24 h For the isolation of pathogenic Staphylococci which give yellow colonies by fermentation of mannitol and phenol red virage. Its high sodium chloride content inhibits the growth of most other species. hStorage: 2 - 25 °C (dehydrated base and ready-to-use medium) hIncubation: 35 ± 2 °C for 18 - 24 h Rich medium used for the cultivation of demanding germs. Its use is recommended for the detection of Staphylococcus aureus in food. hStorage: 2 - 25 °C (dehydrated base and ready-to-use medium) hIncubation: 35 °C - 37 °C for 24 - 48 h Rich medium used for the cultivation of demanding germs. Its use is recommended for the detection of Staphylococcus aureus in food. hStorage: 2 - 25 °C hIncubation: 35 °C ± 2 °C, under atmosphere 5-10 % CO2 for 24 - 72 h Medium for the detection of E.coli and coliforms in the water industry according to ISO 9308 and ISO 11133. hStorage: 2 - 25 °C hIncubation: 36 °C ± 2 °C for 21 ± 3 h For the isolation of Staphylococci in food. It contains ammonium sulphate to detect gelatinase activity (Stone's reaction). hStorage: 2 - 25 °C hIncubation: 30 ± 2 °C for 18 - 48 h For the detection and enumeration of Escherichia coli and coliforms in water by the membrane filtration technique. hStorage: 2 - 25 °C (dehydrated base and ready-to-use medium) hIncubation: 36 ± 2 °C and 44 ± 4 °C for 21 ± 3 h Microbiology Cetrimide (agar) Chapman (agar) Chapman stone (agar) Chapman TTC (lactose agar with tergitol 7) Brain heart (broth) (Brain heart Infusion) Brain heart (agar) Cat. No. Description Pack. € 777217 Dehydrated ketrimide agar medium - Condalab 500 g N/A - Cat. No. Description Pack. € 777307 Dehydrated medium for Chapman agar - Condalab 500 g N/A - Cat. No. Description Pack. € 777218 Chapman stone agar dehydrated medium - Condalab 500 g N/A - Cat. No. Description Pack. € 777196 Dehydrated Medium Brain Heart Agar - Condalab 500 g N/A - Cat. No. Description Pack. € 778391 Chromogenic agar medium for E.coli and coliform detection - Condalab 500 g N/A - Cat. No. Description Pack. € 777425 Dehydrated medium for Chapman TTC agar - Condalab 500 g N/A - Cat. No. Description Pack. € 777824 Dehydrated medium heart brain broth according to ISO 6888 / 11133 - Condalab 500 g N/A - 777197 Dehydrated medium heart and brain broth - Condalab 500 g N/A - Cat. No. Description Pack. € 693319 100 ml bottle of ketrimide agar - ClearLine® 10 N/A - Cat. No. Description Pack. € 993988 100 ml bottle of Chapman Agar - ClearLine® 10 N/A - 778495 100 ml bottle of Chapman Agar - Condalab 150 N/A - 693444 200 ml bottle of Chapman Agar - ClearLine® 6 N/A - Dehydrated base Dehydrated base Ready-to-use media Ready-to-use media h1% TTC (to be reconstituted in 5 ml of sterile distilled water) Cat. No. Description Pack. € 777802 Supplement TTC 1 % 10 vials for 500 ml N/A - Cat. No. Description Pack. € 693327 100 ml bottle of Tergitol 7 agar - ClearLine® 10 N/A - Cat. No. Description Pack. € 693353 100 ml bottle of heart and brain broth ISO 6888 - ClearLine® 10 N/A - Supplement Ready-to-use media Ready-to-use media Dehydrated base Dehydrated bases E. coli - Coliform (chromogenic agar) - Chromogenic coliform agar (CCA) E.coli: dark blue to purple colonies Total coliforms: salmon-red colonies + other colonies DG18 See DG18 agar

MICROBIOLOGY Media Microbiology Reagents - Chemicals 569 All prices are indicative and exclude VAT. Visit www.dulis.nl For the detection of slow growing germs. Can be used with or without the addition of blood. hStorage: 2 - 25 °C (dehydrated medium and ready-to-use medium) hIncubation: 35 °C ± 2 °C, under atmosphere 5-10 % CO 2 for 48 h For multiple flora detection. hStorage: 2 - 25 °C For multiple flora detection. hStorage: 2 - 25 °C For multiple flora detection. The addition of Tween 80 allows for neutralisation and better homogenisation of certain preparations. hStorage: 2 - 25 °C For the determination of antiseptics and disinfectants. hStorage: 2 - 8 °C hIncubation: 37 °C for 48 h Recommended for the detection and enumeration of yeasts and moulds in food and feed products with an aw › 0.95. hStorage: 2 - 8 °C hIncubation: 25 °C for 2-5 days Microbiology Columbia (agar) Deoxycholate (agar) DRBC (Agar) - Dichloran Rose Bengal Chloramphenicol Deoxycholate Lactose (agar) Dextrose (agar) D.R.C.M. - Differential Reinforced Clostridium Broth Thinner pH 7 Thinner pH 7 with neutralizer Thinner pH 7 with Tween 80 (1%) Cat. No. Description Pack. € 777223 Columbia dehydrated medium USP / ISO 10272 / ISO 11133 / European Pharmacopoeia - Condalab 500 g N/A - Cat. No. Description Pack. € 693354 Columbia Agar - ClearLine® - 100 ml bottle 10 N/A - Cat. No. Description Pack. € 693360 100 ml bottle of DRBC agar - ClearLine® 10 N/A - Cat. No. Description Pack. € 777230 Dehydrated medium deoxycholate citrate lactose sucrose agar (D.C.L.S.) - Condalab 500 g N/A - Cat. No. Description Pack. € 777231 Dehydrated deoxycholate agar medium - Condalab 500 g N/A - Cat. No. Description Pack. € 777233 Dehydrated medium deoxycholate lactose agar - Condalab 500 g N/A - Cat. No. Description Pack. € 693426 9 ml tube of pH 7 thinner - ClearLine® 100 N/A - Cat. No. Description Pack. € 777234 Dehydrated Medium Dextrose Agar - Condalab 500 g N/A - Cat.No. Description Pack. € 693427 Thinner in 9ml tube, pH 7 with neutralizer - ClearLine® 100 N/A - 693446 Thinner in 200 ml bottle, pH 7 with neutralizer - ClearLine® 6 N/A - 693442 Thinner in 300 ml bottle, pH 7 with neutralizer - ClearLine® 4 N/A - Cat.No. Description Pack. € 693309 Thinner in 9 ml tube, pH 7 with Tween 80 (1%) - ClearLine® 100 N/A - 693398 Thinner in 90 ml bottle, pH 7 with Tween 80 (1%) - ClearLine® 10 N/A - 693359 Thinner in 100 ml bottle, pH 7 with Tween 80 (1%) - ClearLine® 10 N/A - Cat. No. Description Pack. € 693397 90 ml bottle of D/E Neutralizing Broth - ClearLine® 10 N/A - 693355 100 ml bottle of D/E Neutralizing Broth - ClearLine® 10 N/A - Cat. No. Description Pack. € 777795 Selective supplement for isolation of Neisseria and Haemophilus - Condalab 5 x 2 bottles N/A - 777528 Selective supplement CNV (Vancomycin/ Colistin/Nystatin) for Neisseria spp isolation - Condalab 10 bottles N/A - 777804 Selective supplement CNT (Vancomycin/ Colistin/Nystatin/Trimethoprim) for Neisseria isolation - Condalab 10 bottles N/A - 777803 Selective supplement VCAT (Vancomycin/ Colistin/Namphotericin/Trimethoprim) for Neisseria isolation - Condalab 10 bottles N/A - 777778 Selective supplement CNA (Colistin/Nalidixic Acid) for the isolation of staphylococci, streptococci and penumococci - Condalab 10 bottles N/A - 777769B Selective supplement for the isolation of Brucella spp - Condalab 30 bottles N/A - Dehydrated base Ready-to-use media Supplements D/E (Dey-Engley) Neutralizing Broth D.C.L.S. (deoxycholate citrate lactose sucrose agar) For the isolation and differentiation of Gram (-) enteric bacilli. hStorage 8 - 15 °C hIncubation: 35 °C ± 2 °C for 18 - 24 h For the differentiation and isolation of gram-negative enterobacteria. hStorage 8 - 15 °C hIncubation: 35 °C ± 2 °C for 18 - 24 h For the differentiation and isolation of gram-negative enterobacteria. hStorage 8 - 15 °C hIncubation: 35 °C ± 2 °C for 18 - 24 h Selective medium for Enterobacteriaceae, Salmonella and Shigella. hStorage 8 - 15 °C hIncubation: 37 °C for 24 and 48 h Non-selective medium for the enumeration of Clostridium by the MPN method in water and food. hStorage: 2 - 25 °C hIncubation: 30 °C for 4 - 7 days Cat. No. Description Pack. € 777835 Dehydrated medium for reinforced differential broth Clostridium - Condalab 500 g N/A -

MICROBIOLOGY Media Microbiology Reagents - Chemicals 570 Microbiology Cat.No. Description Pack. € 693462 2 ml tube of sterile physiological water (0.85%) - ClearLine® 6 N/A - 693423 2 ml tube of sterile physiological water (0.85%) - ClearLine® 100 N/A - Cat. No. Description Pack. € 693459 225 ml bottle of buffered peptone water + Tween 80 (4 g/l) - ClearLine® 6 N/A - Sterile distilled water Alkaline peptone water Buffered peptone water with neutralizer Buffered peptone water double concentration Sterile physiological water (0.85%) Buffered peptone water + tween 80 (4 g/l) Buffered peptone water For dilutions or making bacterial suspensions. hStorage 2 - 25 °C hMaximum conductivity 10 µS/cm For the enrichment of Vibrio in food samples, water. hStorage: 2 - 25 °C (dehydrated medium and ready-to-use medium) hIncubation: 35 ± 2 °C for 24 h Thinner used for the preparation of food samples. In particular for pre-enrichment and for the recovery of salmonella before selective enrichment and isolation. The addition of Tween 80 allows for neutralisation and better homogenisation of certain preparations. hStorage: 2 - 25 °C hIncubation: 37 °C for 24 h Thinner used for the preparation of food samples. The double buffer concentration allows it to be used with highly acidic or alkaline products. hStorage: 2 - 25 °C hIncubation: depending on the protocol Isotonic diluent used for diluting or making bacterial suspensions. hStorage: 2 - 25 °C For the recovery of salmonella from foods with selective enrichment and isolation. hStorage: 2 - 25 °C hIncubation: 37 °C for 24 h Thinner used for the preparation of food samples. In particular, it is used as a non-selective pre-enrichment medium for the detection of Salmonella according to standard 6579, as a diluent for the enumeration of microorganisms according to standard 6887, and as a diluent for the enumeration of Listeria monocytogenes according to standard EN ISO 11290-2. hStorage: 2 - 25 °C (dehydrated medium and ready-to-use medium) hIncubation: 37 ± 2 °C for 24 h Dehydrated medium Dehydrated medium Ready-to-use media Ready-to-use media Cat. No. Description Pack. € 777171 Dehydrated medium alkaline peptone water - Condalab 500 g N/A - Cat. No. Description Pack. € 693310 10 ml tube of sterile distilled water - ClearLine® 100 N/A - Cat. No. Description Pack. € 693458 225 ml bottle of buffered peptone water with neutralizer (10%) - ClearLine® 6 N/A - Cat.No. Description Pack. € 693335 225 ml bottle of buffered peptone water double concentratio - ClearLine® 6 N/A - Cat.No. Description Pack. € 777208 Dehydrated medium for buffered peptone water - Condalab 500 g N/A - Cat. No. Description Pack. € 693428 9 ml tube of alkaline peptone water - ClearLine® 100 N/A - 693456 225 ml bottle of alkaline saline peptone water - ClearLine® 6 N/A - Cat. No. Description Pack. € 693429 9 ml tube of Buffered Peptone Water - ClearLine® 100 N/A - 693400 90 ml bottle of buffered peptone water - ClearLine® 10 N/A - 994511 5 litre pouch of buffered peptone water - ClearLine® 55 N/A -

MICROBIOLOGY Media Microbiology Reagents - Chemicals 571 All prices are indicative and exclude VAT. Visit www.dulis.nl Escherichia coli Microbiology EC (Broth) EE (Broth) Elliker (broth) Endo (broth) Endo (agar) Enterobacter sakazakii (chromogenic agar) E.coli coliform (chromogenic agar) E.coli O157 (chromogenic agar) E.coli Enterobacteriaceae (chromogenic agar) For the detection and enumeration of coliforms in water and food according to ISO 11133 / ISO 7251. hStorage: 2 - 25 °C hIncubation: 44 ± 1 °C for 24 ± 2 - 48 ± 2 h Medium for the detection of E.coli and other coliforms in water (membrane filtration) and food samples. hStorage: 2 - 25 °C hIncubation: 35 °C ±2 °C for 18 - 24 h Selective medium for the detection of E.coli O157:H7 in industries. hStorage: 2 °C - 8 °C hIncubation: 35 °C ± 2 °C for 18 - 24 h hCefixime tellurite. To be reconstituted in 5 ml sterile distilled water Chromogenic medium for the simultaneous detection of E.coli and Enterobacteriaceae in the food industry. hStorage: 2 °C - 8 °C hIncubation: 35 °C ± 2 °C for 18 - 24 h E.coli: dark blue - blue-green colonies Enterobacteria: magenta colonies Supplement Cat. No. Description Pack. € 777240 Dehydrated medium for EC broth - Condalab 500 g N/A - Cat. No. Description Pack. € 777241 Dehydrated broth medium EE (according to ISO 21528-1:2005) - Condalab 500 g N/A - Cat. No. Description Pack. € 777242 Dehydrated medium for Elliker broth - Condalab 500 g N/A - Cat. No. Description Pack. € 777238 Chromogenic medium agar for E.coli and coliform detection - Condalab 500 g N/A - Cat. No. Description Pack. € 777836 Chromogenic Base Medium for E.coli O157:H7 - Condalab 500 g N/A - Cat. No. Description Pack. € 777771 Cefixime tellurite supplement - Condalab 10 bottles for 500 ml N/A - Cat. No. Description Pack. € 777916 Chromogenic agar medium for E.coli - Enterobacteria - Condalab 500 g N/A - For the detection and enumeration of enterobacteria by the MPN method. hStorage 2 - 8 °C hIncubation: 37 ± 1 °C for 24 ± 2 h For the cultivation of streptococci and lactobacilli in dairy products. hStorage: 2 - 25 °C hIncubation: 32 ± 2 °C for 18 - 48 h, except for streptococcus cremoris incubation at 30 ± 2 °C For the identification of enterobacteria. hStorage: 2 - 25 °C hIncubation: 35 °C ± 2 °C for 24 h ± 2 h Cat. No. Description Pack. € 778426 Endo Broth Dehydrated Medium - Condalab 500 g N/A - Cat. No. Description Pack. € 777243 Endo agar dehydrated medium - Condalab 500 g N/A - Cat. No. Description Pack. € 777838 Dehydrated medium for Enterobacter sakazakii chromogenic agar - Condalab 500 g N/A - hEthanol (20 ml) hFuchsin basic Cat. No. Description Pack. € 4146012-CER Ethanol - RPE for analysis 1 litre N/A - 452842-CER Basic Fuchsin - RPE for analysis 25 g N/A - Supplements For the determination of coliforms in water, dairy products and food in general. hStorage 8 - 15 °C hIncubation: 35 °C ± 2 °C for 18 - 24 h For the presumption and isolation of Enterobacter sakazakii. hStorage 8 - 15 °C hIncubation: 44 °C for 24 h Green/blue colonies: E. sakazakii Red/coloured colonies: E. coli

MICROBIOLOGY Media Microbiology Reagents - Chemicals 572 ESTY (agar) Ewing malonate (broth) Yeast extract Malt extract Eugon LT100 (broth) Fraser Half Fraser Lactose gelatin Cat. No. Description Pack. € 777248 Dehydrated medium for ESTY agar - Condalab 500 g N/A - Cat. No. Description Pack. € 777251 Dehydrated medium for modified Ewing malonate broth - Condalab 500 g N/A - Cat. No. Description Pack. € 777474 Yeast extract - Condalab 500 g N/A - Cat. No. Description Pack. € 777462 Malt extract - Condalab 500 g N/A - For the enumeration of thermophilus streptococci in yoghurt. hStorage: 2 - 25 °C hIncubation: 35 ± 2 °C for 24 - 48 h For the differentiation of coliforms. hStorage: 2 - 25 °C hIncubation: 35 ± 2 °C for 18 - 48 h An ingredient used as a source of nitrogen in media. hStorage: 2 - 25 °C An ingredient used as a source of nitrogen in media. hStorage: 10 - 35 °C For the enrichment of micro-organisms. Recommended for the enumeration of aerobic mesophilic bacteria, yeast and moulds and Pseudomonas aeruginosa, Staphylococcus aureus. hStorage: 2 - 25 °C (Dehydrated medium and ready-to-use medium) hIncubation: 30 - 35 °C for 24 to 72 h For the detection and enumeration of Listeria in food products. hStorage: 2 - 25 °C (for the base) and 2 - 8 °C (for the ready-to-use medium) hIncubation: 30 °C ± 1 °C for 24 hours ± 2 hours For the detection and enumeration of Listeria in food products. hStorage: 2 - 25 °C (for the base) and 2 - 8 °C (for the ready-to-use medium) hIncubation (according to standard 11290): 30 °C for 25 hours ± 1 hour For confirmation of clostridium perfringens. hStorage: 2 - 25 °C hIncubation: 35 ± 2 °C for 18 - 48 h Dehydrated medium Dehydrated medium Dehydrated medium Ready-to-use media Supplement Supplement Ready-to-use media Ready-to-use media Cat. No. Description Pack. € 778403 Dehydrated medium for eugon broth LT100 - Condalab 500 g N/A - Cat. No. Description Pack. € 777547 Fraser Broth Dehydrated Medium - ISO 11133 / ISO 11290 compliant - Condalab 500 g N/A - Cat. No. Description Pack. € 777852 Fraser demi broth dehydrated medium - ISO 11133 / ISO 11290 compliant - Condalab 500 g N/A - Cat. No. Description Pack. € 777256 Dehydrated medium gelatine lactose broth - Condalab 500 g N/A - Cat. No. Description Pack. € 777782 Ferric ammonium citrate supplement - Condalab 10 bottles N/A - Cat. No. Description Pack. € 777782 Ferric ammonium citrate supplement - Condalab 10 bottles N/A - Cat. No. Description Pack. € 693409 10 ml tube of Fraser broth in accordance with ISO 11290-1 - ClearLine® 100 N/A - Cat. No. Description Pack. € 693460 225 ml bottle of Half Fraser broth - ClearLine® 6 N/A - 994512 5 litres pouch of Half Fraser broth - ClearLine® 55 N/A - Cat. No. Description Pack. € 693300 9 ml tube of eugon broth LT100 - ClearLine® 100 N/A - 693401 Eugon Broth 90 ml bottle LT100 - ClearLine® 10 N/A - hFerric ammonium citrate. To be reconstituted in 5 ml of warm sterile distilled water hFerric ammonium citrate. To be reconstituted in 5 ml of warm sterile distilled water Microbiology ESTY (Broth) Cat. No. Description Pack. € 777247 Dehydrated medium for ESTY broth - Condalab 500 g N/A - For the isolation and enumeration of lactic streptococci in yoghurt and dairy products. hStorage: 2 - 25 °C hIncubation: 35 ± 2 °C for 24 - 48 h

MICROBIOLOGY Media Microbiology Reagents - Chemicals 573 All prices are indicative and exclude VAT. Visit www.dulis.nl Cat. No. Description Pack. € 777263 Dehydrated medium Hektoen Agar - Condalab 500 g N/A - Cat. No. Description Pack. € 777265 Dehydrated medium I.T.C. broth - Condalab 500 g N/A - Cat. No. Description Pack. € 777785 Supplement for I.T.C. broth - Condalab 10 bottles for 500 ml N/A - Cat. No. Description Pack. € 693356 DG18 Agar 100 ml bottle - ClearLine® 10 N/A - Cat. No. Description Pack. € 777816 Dehydrated Actinomycete Isolation Agar Medium - Condalab 500 g N/A - Supplement hTo be reconstituted in 8 ml sterile distilled water hStorage: 2 - 8 °C Microbiology For the culture of anaerobic germs, especially Clostridium. hStorage 8 - 15 °C hIncubation at 35 °C ± 2 °C for 18 - 48 h For the detection and enumeration of yeasts and moulds in dry products with an aw ‹ 0.95. hStorage: 2 - 8 °C in the dark hIncubation: 25 °C for 5 days in a humid aerobic atmosphere For the isolation and cultivation of actinomycetes in soil and water. hStorage: 2 - 25 °C hIncubation: 30 °C ± 2 °C for 72 h Broth for selection and enrichment of yersinia enterolitica. hStorage: 2 - 25 °C hIncubation: 25 ± 1 °C for 44 ± 4 h Cat.No. Description Pack. € 777174 Dehydrated medium anaerobic agar - Condalab 500 g N/A - Anaerobic agar DG18 agar Baird-Parker Agar Middlebrook Agar PCA agar Wilson Blair Agar Giolotti-Cantoni (broth) Hektoen (agar) Blood agar Campylobacter agar Cetrimide agar Chapman agar Salmonella chromogenic agar Brain heart agar Columbia agar DRBC agar See Baird-Parker agar See middlebrook agar See PCA See Wilson Blair agar See campylobacter See ketrimide agar See chapman agar See Salmonella chromogenic agar See Heart brain agar See columbia agar See DRBC agar I.T.C. - Irgasan Ticarcillin and potassium Chlorate broth To differentiate Shigella from Escherichia coli. hStorage 8 - 15 °C hIncubation at 35 °C ± 2 °C for 7 days Cat.No. Description Pack. € 777169 Dehydrated medium acetate differential agar Condalab 500 g N/A - For the determination of haemolytic germs and confirmation of Bacillus cereus and Listeria monocytogenes. hStorage 8 - 15 °C hIncubation at 35 °C ± 2 °C for 18 - 48 h for Listeria monocytogenes hIncubation at 30 °C for 24 h for Bacillus cereus Cat.No. Description Pack. € 777823 Blood agar dehydrated medium (ISO7932) - Condalab 500 g N/A - Cat. No. Description Pack. € 777257 Dehydrated medium Giolotti broth - Cantoni - Condalab 500 g N/A - 777258 Dehydrated medium Giolotti broth - Cantoni - ISO 11133 - Condalab 500 g N/A - Enrichment medium used for the detection of Staphylococcus aureus in foodstuffs. hStorage: 2 - 25 °C hIncubation: 35 ± 2 °C for 40 - 48 h hAccording to ISO 11133: 37 ± 1 °C for 24 ± 2 - 48 ± 2 h Di f ferent ial select i ve medium for enteropathogenic bacteria, especially salmonella and shigella. The composition of the medium allows the differentiation o f co l on i e s r ap i d l y f e rmen t i ng one of the 3 sugars (turning from blue to sa lmon- red) and/or produc ing H2S (black centre). hStorage: 2 - 25 °C hIncubation: 37 ± 1 °C for 20 - 24 h Acetate differential agar Actinomycede Isolation agar glycerol

MICROBIOLOGY Media Microbiology Reagents - Chemicals 574 Egg yolk (emulsion) Lactose (broth) Lactose sulphite (broth) Lauryl sulphate (broth) - Lauryl Tryptose Broth (LTB) Lauryl sulphate (agar) Chromogenic Lauryl Sulphate (broth) Kligler Hajna (agar) Kligler iron (agar) Koser citrate (broth) Egg yolk with potassium tellurite KAA presumption (broth) KAA confirmation (agar) Cat. No. Description Pack. € 777780 100 ml bottle of egg yolk emulsion - Condalab 1 N/A - Cat. No. Description Pack. € 777848 Dehydrated medium for lauryl sulphate chromogenic broth - Condalab 500 g N/A - Cat. No. Description Pack. € 777278 Dehydrated medium lactose broth - Condalab 500 g N/A - Cat. No. Description Pack. € 777279 Dehydrated medium for lactose sulphite broth - Condalab 500 g N/A - Cat. No. Description Pack. € 693436 Kligler Hajna Agar Slant Tube - ClearLine® 100 N/A - Cat. No. Description Pack. € 777281 Dehydrated medium for lauryl sulfate broth - Condalab 500 g N/A - Cat. No. Description Pack. € 777280 Dehydrated medium for lauryl sulfate agar - Condalab 500 g N/A - Cat. No. Description Pack. € 777275 Dehydrated medium Kligler iron agar - Condalab 500 g N/A - Cat. No. Description Pack. € 777276 Dehydrated medium Koser citrate broth - Condalab 500 g N/A - Cat. No. Description Pack. € 777800 100 ml bottle of egg yolk emulsion with potassium telliuric - Condalab 1 N/A - Cat. No. Description Pack. € 777267 Dehydrated medium KAA presumption broth - Condalab 500 g N/A - Cat. No. Description Pack. € 777266 Dehydrated medium KAA confirmation agar - Condalab 500 g N/A - Used as a supplement in many culture media for the isolation or identification of Bacillus and Staphylococcus. hStorage: 8 - 14 °C For the culture of coliforms and salmonella in water, food, dairy products. hStorage: 2 - 25 °C hIncubation: 35 ± 2 °C for 18 - 24 h Enrichment medium for the simultaneous detection of total coliforms and Escherichia coli in water, food, dairy products. hStorage: 2 °C - 8 °C hIncubation: 35 °C ± 2 °C for 18 - 24 h. Control of the tubes under UV 366 nm lamp. Blue light indicates the presence of E. coli For the culture of coliforms and salmonella in water, food, dairy products. hStorage: 2 - 25 °C for the dehydrated base and 2 - 8 °C for the ready-to-use tubes hIncubation: 46 °C for 18 - 24 h Used to detect coliforms in food, water, milk and dairy products. The use of inoculum larger than 1 ml requires the use of double strength lauryl sulphate broth to ensure that sufficient nutrients are maintained. hStorage: 2 - 25 °C hAccording to ISO 4831: 30 °C ou 37 °C for 24 h hAccording to ISO 7251: 37 °C for 48 h, examining gas production after 24 h and 48 h Selective medium for the detection of coliforms in water, dairy products and seafood. hStorage: 2 - 25 °C hIncubation: 35 ± 2 °C for 24 - 48h For the presumptive identification of enterobacteria based on the fermentation of glucose, lactose, sucrose and the production of gas and H2S. For the detection of salmonella in food and dairy products. hStorage: 2 - 25 °C in the dark hIncubation: 37 °C for 18 - 24 h Medium for the detection of yersinia enterolitica. hStorage: 2 - 25 °C hIncubation: 30 °C for 18 - 24 h For the differentiation of escherichia coli from enterobacteria based on the use of citrate. hStorage: 2 - 25 °C hIncubation: 35 ± 2 °C for 18 - 24 h For the preparation of the Baird Parker according to ISO 6888. hStorage: 8 - 14 °C For the presumption and detection of intestinal enterococci in foods according to Mossel. hStorage: 2 - 25 °C hIncubation: 35 ± 2 °C for 24 - 48 h For the isolation and confirmation of intestinal enterococci in foods according to MOSSEL. hStorage: 2 - 25 °C hIncubation: 35 ± 2 °C for 24 - 48 h Microbiology Enterococcus faecalis hAfter heating the tube to 100 °C and allowing it to cool for 5 minutes, add 0.5 ml of a 12 g/l solution of sodium metabisulphite and 0.5 ml of a 10 g/l solution of ammoniacal ferric citrate sterilised by filtration through a 0.45 µm membrane Dehydrated medium Supplement Ready-to-use media Cat. No. Description Pack. € 693314 8 ml tube of Durham broth lactose sulphite - ClearLine® 100 N/A - 693408 9 ml tube with Durham bell broth lactose sulphite - ClearLine® 100 N/A -

MICROBIOLOGY Media Microbiology Reagents - Chemicals 575 All prices are indicative and exclude VAT. Visit www.dulis.nl LB Lennox formulation (agar) LB Lennox base (broth) Luria LB base (agar) LB broth (Luria) Miller-Luria LB base (agar) Miller-Luria LB base (broth) hStorage: 2 - 25 °C (dehydrated medium) 2 - 8 °C (for vials) hIncubation: 35 °C ± 2 °C for 18 - 24 h hStorage: 2 - 25 °C (dehydrated medium) 2 - 8 °C (for vials) hIncubation: 35 °C ± 2 °C for 18 - 24 h hStorage: 2 - 25 °C (dehydrated medium) 2 - 8 °C (for vials) hIncubation: 35 °C ± 2 °C for 18 - 24 h hStorage: 2 - 25 °C (dehydrated medium) 2 - 8 °C (for vials) hIncubation: 35 °C ± 2 °C for 18 - 24 h hStorage: 2 - 25 °C (dehydrated medium) 2 - 8 °C (for vials) hIncubation: 35 °C ± 2 °C for 18 - 24 h hStorage: 2 - 25 °C (dehydrated medium) 2 - 8 °C (for vials) hIncubation: 35 °C ± 2 °C for 18 - 24 h Microbiology A medium recommended in molecular microbiology for the culture of Bacillus subtilis, Daptobacter and recombinant strains of Escherichia coli. Broth recommended in molecular microbiology for the cultivation of Bacillus subtilis, Daptobacter and recombinant strains of Escherichia coli. A medium recommended in molecular microbiology for the culture of Bacillus subtilis, Daptobacter and recombinant strains of Escherichia coli. A medium recommended in molecular microbiology for the culture of Bacillus subtilis, Daptobacter and recombinant strains of Escherichia coli. A medium recommended in molecular microbiology for the culture of Bacillus subtilis, Daptobacter and recombinant strains of Escherichia coli. A medium recommended in molecular microbiology for the culture of Bacillus subtilis, Daptobacter and recombinant strains of Escherichia coli. h Composition (g/l): h Composition (g/l): h Composition (g/l): h Composition (g/l): h Composition (g/l): h Composition (g/l): Tryptone 10 Sodium chloride 5 Yeast extract 5 Agar 15 Tryptone 10 Sodium chloride 5 Yeast extract 5 - - Tryptone 10 Sodium chloride 0.5 Yeast extract 5 Agar 15 Tryptone 10 Sodium chloride 0.5 Yeast extract 5 - - Tryptone 10 Sodium chloride 10 Yeast extract 5 Agar 15 Tryptone 10 Sodium chloride 10 Yeast extract 5 - - Cat. No. Description Pack. € 777490 Dehydrated medium LB Lennox agar - Condalab 500 g N/A - 693363 LB Lennox Agar - 100 ml bottle - ClearLine® 10 N/A - Cat. No. Description Pack. € 777491 Dehydrated medium LB Lennox Broth - Condalab 500 g N/A - 777921 Dehydrated medium LB Lennox Broth - Condalab 1 kg N/A - 693362 LB Lennox Broth - 100 ml bottle - ClearLine® 10 N/A - Cat. No. Description Pack. € 777492 Dehydrated medium agar LB formulation Luria (Miller modified) - Condalab 500 g N/A - 693365 100 ml bottle of Luria LB Agar - ClearLine® 10 N/A - Cat. No. Description Pack. € 777493 Dehydrated medium LB formulation LURIA broth (modified miller) - Condalab 500 g N/A - 693364 100 ml bottle of Luria LB broth - ClearLine® 10 N/A - Cat. No. Description Pack. € 777494 Dehydrated medium Luria agar (LB Miller formulation) - Condalab 500 g N/A - 693367 Luria Agar (Miller's LB Broth Base) - 100 ml bottle - ClearLine® 10 N/A - Cat. No. Description Pack. € 777495 Dehydrated medium Luria broth (LB Miller formulation) - Condalab 500 g N/A - 777920 Dehydrated medium Luria broth (LB Miller formulation) - Condalab 1 kg N/A - 693366 LB Miller Broth base 100 ml Bottle - ClearLine® 10 N/A -

MICROBIOLOGY Media Microbiology Reagents - Chemicals 576 Microbiology sLB (broth) Lysine iron (agar) Lysine decarboxylase (broth) ISO 10273 Lysine decarboxylase (broth) ISO 6579 M17 (Agar) Letheen (broth) Modified Letheen (broth) Cat. No. Description Pack. € 777502 Dehydrated medium for buffered sLB broth - Condalab 500 g N/A - Cat. No. Description Pack. € 777296 Dehydrated medium lysine iron agar - Condalab 500 g N/A - Cat. No. Description Pack. € 777294 Dehydrated medium lysine decarboxylase broth ISO 10273 - Condalab 500 g N/A - Cat. No. Description Pack. € 777295 Dehydrated medium lysine decarboxylase broth ISO 6579 - Condalab 500 g N/A - Cat. No. Description Pack. € 777297 Dehydrated medium M17 agar - Condalab 500 g N/A - Cat. No. Description Pack. € 693430 9 ml tube Letheen broth - ClearLine® 100 N/A - 693411 10 ml tube Letheen broth - ClearLine® 100 N/A - 693323 100 ml bottle Letheen broth - ClearLine® 10 N/A - For bacterial growth and leads to high yields of low copy number plasmid. Used in molecular biology. hStorage: 2 - 25 °C hIncubation: 35 °C ± 2 °C for 24 h; 48 and 72 h Used in the cosmetics industry to neutralise the action of conservatives before microbiological examination or to determine the bactericidal activity of quaternary ammoniums. hStorage 2 - 8 °C To neutralise the action of conservatives before microbiological examination. hStorage: 2 - 25 °C (for dehydrated base) and 2 - 8 °C (for ready-to-use media) Cat. No. Description Pack. € 777284 Dehydrated medium for modified letheen broth - Condalab 500 g N/A - Cat. No. Description Pack. € 693431 9 ml tube of modified letheen broth - ClearLine® 100 N/A - 693402 90 ml bottle of modified letheen broth - ClearLine® 10 N/A - 693447 200 ml bottle of modified letheen broth - ClearLine® 6 N/A - Dehydrated medium Ready-to-use media Levine (agar) Listeria (agar) Cat. No. Description Pack. € 777285 Dehydrated medium Levine agar (E.M.B.) - Condalab 500 g N/A - Cat. No. Description Pack. € 777289 Dehydrated medium for Listeria chromogenic agar according to Ottaviani and Agosti (ALOA) - Condalab 500 g N/A - Cat. No. Description Pack. € 777790 Lipase C supplement - Condalab 10 bottles for 500 ml N/A - 777789 Cycloheximide + polymexin B sulphate + Ceftazimidime + nalidixic acid supplement - Condalab 10 bottles for 500 ml N/A - Low selectivity medium for the differentiation of lactose-positive and lactose-negative Enterobacteriaceae contained in food and dairy products. hStorage: 2 - 25 °C hIncubation: 35 ± 2 °C for 18 - 24 h Medium for the detection and enumeration of Listeria monocytogenes. hStorage: 2 - 25 °C hIncubation: 35 ± 2 °C for 18 - 24 h Escherichia coli Supplements To be reconstituted in 5 ml of a water/acetone mixture (1/1). For 500 ml of medium. h1 Bottle of Lipase C substrate h1 Bottle containing: 50 mg Cycloheximide + 10 mg Ceftazidime + 0 mg Nalidixic acid + 38350 IU Polymyxin B Listeria monocytogenes Listeria innocua Allows differentiation of Salmonella arizonae by studying lysine decarboxylation. hStorage: 2 - 25 °C hIncubation: 35 ± 2 °C for 18 - 48 h For the identification of micro-organisms, especially enteric bacilli, on the basis of their ability to decarboxylate lysine. hStorage: 2 - 25 °C hIncubation: 35 ± 2 °C for 18 - 48 h For biochemical confirmation of Salmonella and Yersina enterocolitica. hStorage: 2 - 25 °C hIncubation: 37 ± 2 °C for 24 ± 3 h For the culture and enumeration of lactic streptococci in milk and dairy products. hStorage: 2 - 25 °C hIncubation: 28 - 30 °C for 24 - 48 h

MICROBIOLOGY Media Microbiology Reagents - Chemicals 577 All prices are indicative and exclude VAT. Visit www.dulis.nl Microbiology Mac Conkey (broth) - according to pharmacopoeia Mac Conkey (agar) - according to pharmacopoeia Cat. No. Description Pack. € 777303 Dehydrated medium Mac Conkey Broth - Condalab 500 g N/A - Cat. No. Description Pack. € 777298 Dehydrated medium Mac agar Conkey - Condalab 500 g N/A - Cat. No. Description Pack. € 693302 100 ml bottle of Mac Conkey Broth - ClearLine® 10 N/A - Cat. No. Description Pack. € 693369 100 ml bottle of Mac ConkeyBroth* - ClearLine® 10 N/A - 693448 100 ml bottle of Mac ConkeyBroth* - ClearLine® 6 N/A - * Not in accordance with the pharmacopoeia For the detection of coliforms in water, milk and pharmaceutical preparations. hStorage: 2 - 25 °C (dehydrated base and ready-to-use medium) hIncubation: 42 - 44 °C for 24 - 48 h Di fferential medium to guide the identification of lactose-fermenting and non-lactose-fermenting Enterobacteriaceae. It is recommended for the detection of escherichia coli in water, food, dairy products and pharmaceutical preparations. hStorage: 2 - 25 °C (dehydrated base and ready-to-use medium) hIncubation hAccording to ISO 21567: 37 ± 1 °C for 20 - 24 h hAccording to ISO 21150: 30 - 35 °C for 24 h Dehydrated base Dehydrated base Ready-to-use media Ready-to-use media Enterobacter aerogenes Escherichia coli Dehydrated base Ready-to-use media Malt (Broth) Malt (Agar) Broth for the isolation and culture of yeasts and moulds. hStorage 2 - 25 °C hIncubation: 30 ± 2 °C for 18 - 72 h Cat.No. Description Pack. € 777305 Dehydrated medium malt extract broth - Condalab 500 g N/A - Recommended for the detection, isolation and enumeration of yeasts and moulds. hStorage: 2 - 25 °C (dehydrated base) - 2 - 8 °C (ready-to-use medium) hIncubation: 25 - 30 °C for 18 - 72 h Cat. No. Description Pack. € 777304 Dehydrated medium malt extract agar - Condalab 500 g N/A - Cat. No. Description Pack. € 693437 Slant tube malt extract agar - ClearLine® 100 N/A - 693370 100 ml bottle malt extract agar - ClearLine® 10 N/A - Dehydrated base Ready-to-use media Mannitol salt Agar (MSA) Mannitol mobility nitrate Marine (Agar) M-EI (chromogenic agar) See Chapman agar For the presumptive identification of Enterobacteriaceae based onmannitol fermentation, germ mobility and nitrate to nitrite reduction. hStorage: 2 - 25 °C (dehydrated base) - 2 - 8 °C (ready-to-use medium) hIncubation: 35 ± 2 °C for 18 - 24 h For the isolation and enumeration of heterotropic marine bacteria. hStorage: 2 - 8 °C hIncubation: 20 - 25 °C for 24 - 72 h For the detection and enumeration of Enterococcus in water by the membrane filtration method. hStorage: 2 - 25 °C hIncubation: 41 ± 0.5 °C for 18 - 24 h Cat. No. Description Pack. € 777306 Dehydrated medium mannitol mobility nitrate - Condalab 500 g N/A - Cat. No. Description Pack. € 693461 10 ml tube mannitol mobility nitrate - ClearLine® 6 N/A - 693412 10 ml tube mannitol mobility nitrate - ClearLine® 100 N/A - Cat. No. Description Pack. € 777309 Dehydrated medium marine agar medium - Condalab 500 g N/A - Cat. No. Description Pack. € 777310Dehydrated medium for chromogenic agar M-EI - Condalab 500 g N/A - Modified M-EI (chromogenic agar) For the isolation and differentiation of Enterococcus faecalis and Enterococcus faecium hStorage: 2 - 8 °C hIncubation: 41 ± 0.5 °C for 18 - 24 h Cat. No. Description Pack. € 778436 Dehydrated medium for modified M-EI chromogenic agar - Condalab 500 g N/A -

MICROBIOLOGY Media Microbiology Reagents - Chemicals 578 Culture Media Dehydrated base Dehydrated base Ready-to-use media Ready-to-use media Supplements Moeller KCn (Broth) MIO - Medium Mobility Indole Ornithine Mossel (Broth) - EE (Broth) Mossel (Bacillus cereus - Agar) MRS deMan,Rogosa, Sharpe (Broth) Cat. No. Description Pack. € 777315 Dehydrated medium Mossel EE broth - 100 ml bottle 500 g N/A - Cat. No. Description Pack. € 693413 9 ml tube of Mossel* broth - ClearLine® 100 N/A - 693371 100 ml bottle of Mossel broth- ClearLine® 10 N/A - * Not in accordance with the pharmacopoeia MKTTn - Müller Kauffman bright green with novobiocin (Broth) Medium used for the selective enrichment of Salmonella. The use of this medium requires the extemporaneous addition of an iodine solution. hStorage: 2 - 25 °C hIncubation: according to ISO 19250 and ISO 11133: 37 °C ± 1 °C for 24 ± 3 h Cat. No. Description Pack. € 777324 Dehydrated medium for MKTTn broth - Condalab 500 g N/A - Supplements For 100 ml of medium hAdd extemporaneously 20 ml of a distilled or demineralised water solution containing 5 g of potassium iodide and 4 g of iodine. Note that the complete medium must be used the same day. Cat. No. Description Pack. € 348454-CER Iodine 100 g N/A - 362405-CER Potassium iodide 250 g N/A - To differentiate enteric bacilli that have the ability to grow in the presence of cyanide. hStorage 2 - 25 °C hIncubation: 35 °C ± 2 °C for 24 - 48 h Cat. No. Description Pack. € 777314 Dehydrated medium Moeller KCn Broth - Condalab 500 g N/A - Semi-solid medium for differentiation of enterobacteria by motility, ornithine decarboxylase activity and indole production. hStorage: 2 - 25 °C hIncubation: 35 °C ± 2 °C for 24 - 48 h Enrichment broth for Enterobacteriaceae according to Mossel is a selective medium recommended for the investigation of Enterobacteriaceae in foods. Complies with the European Pharmacopoeia. hStorage: 2 - 30 °C (dehydrated base) - 2 - 8 °C (ready-to-use medium) hIncubation: 37 °C for 24 - 48 h Cat. No. Description Pack. € 777313 Dehydrated medium Indole ornithine mobility - Condalab 500 g N/A - hFor indole detection Cat. No. Description Pack. € 777535 KOVAC reagent - Condalab 100 ml N/A - 435922-CER KOVAC reagent 100 ml N/A - Recommended for the detection and enumeration of spores and vegetative forms of Bacillus cereus in foodstuffs. hStorage: 2 - 25 °C hIncubation: 35 °C ± 2 °C for 24 - 40 h Supplements For 900 ml of medium. hEgg yolk emulsion: 100 ml hPolymixin B*: 100000 UI Cat. No. Description Pack. € 777183 Dehydrated medium Bacillus cereus selective agar according to Mossel (MYP) - Condalab 500 g N/A - Cat. No. Description Pack. € 777780 100 ml bottle Egg yolk emulsion - condalab 1 N/A - 777767 Polymixin B* supplement - condalab 10 vials for 500 ml N/A - * To be reconstituted in 5 ml sterile distilled water Bacillus cereus For lactobacillus culture. hStorage: 2 - 25 °C (for dehydrated base) and 2 - 8 °C (for ready-to-use media) hIncubation: 35 °C for 3 days or 30 °C for 5 days in a CO2enriched atmosphere Cat. No. Description Pack. € 777317 Dehydrated broth medium MRS - Condalab 500 g N/A - Cat. No. Description Pack. € 693432 9 ml tube of MRS broth - ClearLine® 100 N/A - 693344 100 ml bottle of MRS broth - ClearLine® 10 N/A -

MICROBIOLOGY Media Microbiology Reagents - Chemicals 579 All prices are indicative and exclude VAT. Visit www.dulis.nl Culture Media Dehydrated base Dehydrated base Ready-to-use media Ready-to-use media MRS deMan,Rogosa, Sharpe (Agar) MRS deMan,Rogosa, Sharpe pH 5.7 (Agar) For lactobacillus culture. The pH 5.4 medium allows the enumeration of Lactobacillus bulgaricus in yoghurt. hStorage: 2 - 8 °C (for dehydrated base and for ready-to-use media) hIncubation: 35 °C for 3 days or 30 °C for 5 days in a CO2enriched atmosphere The pH 5.7 medium allows the enumeration of mesophilic lactic acid bacteria hStorage: 2 - 8 °C (for dehydrated base and for ready-to-use media) hIncubation: 35 °C for 3 days or 30 °C for 5 days in a CO2enriched atmosphere Cat. No. Description Pack. € 777316 Dehydrated medium MRS agar - Condalab 500 g N/A - Cat. No. Description Pack. € 777858 Dehydrated medium MRS agar low pH ISO - Condalab 500 g N/A - Cat. No. Description Pack. € 693372 100 ml bottle of MRS agar pH 5.7 ClearLine® 10 N/A - Cat. No. Description Pack. € 693449 200 ml bottle of MRS agar - ClearLine® 6 N/A - MRVP (Methyl Red - Voges Proskauer) Clarks and Lubs Medium MSRV Modified Semisolid Rappaport Vassiliadis (Broth) For the differentiation of Escherichia coli from Entobacteria hStorage: 2 - 25 °C hIncubation: - For the methyl red test: 35 ± 2 °C for 3 to 5 days (Tube to be incubated before testing) - For the Voges Proskauer test: 35 ± 2 °C For the detection of mobile salmonella species according to ISO 6579 hStorage: 2 - 25 °C hIncubation: 41.5 °C for 24 h Cat. No. Description Pack. € 777320 Dehydrated medium MR-VP from Clark and Lubs - Condalab 500 g N/A - Cat. No. Description Pack. € 3390062.5 ml bottle of alpha naphthol + 2.5 ml bottle of potassium hydroxide for the VP test 10 + 10 N/A - Reagent required h5 drops of a 0.4% methyl red solution for the MR test Modified Rappaport Vassiliadis broth Cat. No. Description Pack. € 777319 Dehydrated medium MSRV medium - Condalab 500 g N/A - Mueller Hinton (Agar) Reference medium for antibiotic and sulphonamide susceptibility testing of germs. hStorage: 2 - 25 °C (for dehydrated base) and 2 - 8 °C (for ready-to-use media) hIncubation: 35 ± 2 °C for 24 h Dehydrated base Ready-to-use media Cat. No. Description Pack. € 777321 Dehydrated medium Mueller Hinton agar - Condalab 500 g N/A - Cat. No. Description Pack. € 693375 100 ml bottle of Mueller Hinton agar - ClearLine® 10 N/A - Supplement hAdd 20 ml/litre of iodide solution and 10 ml/litre of 0.1% bright green solution. To be used the same day Mueller Kauffman tetrathionate (Broth) For the selective enrichment of salmonella in food. hStorage: 2 - 25 °C hIncubation: 42 - 43 °C for 6 - 24 h Cat. No. Description Pack. € 777325 Dehydrated medium Mueller Kauffman Tetrathionate Broth - Condalab 500 g N/A - The neutralizer mixture is added to the pre-enrichment medium whenever the presence of disinfectant solution is known or suspected. As a general rule, this mixture should be used at a rate of 10% of the final volume of the pre-enrichment medium. Neutralizer hStorage 15 - 25 °C in the dark Formula in g/l Disodium phosphate 100.8 L-Histidine HCL 1 Sodium thiosulphate 7.8 Tween 80 30 Lecithin 3 Distilled water 857.4 Cat. No. Description Units/ box €/ box 693422 ClearLine® Neutralizer - Tube 25 ml 100 N/A - Universal neutralizer The neutralizer mixture is added to the pre-enrichment medium whenever the presence of disinfectant solution is known or suspected. As a general rule, this mixture should be used at a rate of 10% of the final volume of the pre-enrichment medium. Cat. No. Description Units/ box €/ box 693329 Universal neutralizer ClearLine® - 100 ml bottle 10 N/A - hStorage 15 - 25 °C in the dark Formula in g/l Disodium phosphate 75.45 Tween 20 20 Sodium thiosulphate 7.8 Tween 80 30 Lecithin 3 Distilled water 950 L-Histidine HCL 1

MICROBIOLOGY Media Microbiology Reagents - Chemicals 580 Culture Media Nutrient agar with sodium chloride ISO 21258 Dehydrated base Dehydrated base Ready-to-use media Ready-to-use media Cat. No. Description Pack. € 777335 Dehydrated medium for O.G.A. agar (O.G.Y.E.) - Condalab 500 g N/A - Cat. No. Description Pack. € 777331 Dehydrated medium for nutrient agar with sodium chloride ISO 21258 - Condalab 500 g N/A - Cat. No. Description Pack. € 693373 100 ml bottle O.G.A. agar (O.G.Y.E.) - ClearLine® 10 N/A - Cat. No. Description Pack. € 693374 100 ml bottle of nutrient agar with sodium chloride - ClearLine® 10 N/A - Cat. No. Description Pack. € 777327 Dehydrated medium nitrate mobility medium - Condalab 500 g N/A - Nitrate mobility (Agar) Harmful bacteria for breweries (Broth) Harmful bacteria for breweries (Agar) Osmophilic (Agar) Ox bile, bacteriological Nutritive (Broth) Nutrient agar Medium for confirmation of clostridium perfringens. hStorage: 2 - 25 °C hIncubation: 35 ± 2 °C for 24 - 48 h For the detection of harmful contaminants in brewing. hStorage: 2 - 25 °C hIncubation: 30 - 35 °C for 4 days For the detection of harmful contaminants in brewing. hStorage: 2 - 25 °C hIncubation: 30 - 35 °C for 4 days For the cultivation of non-demanding germs hStorage: 2 - 25 °C hIncubation: 35 ± 2 °C for 18 - 48 h Cat. No. Description Pack. € 777863 Dehydrated medium for Nocive Brewers bacteria Broth Agar base modified - Condalab 500 g N/A - Cat. No. Description Pack. € 777862 Dehydrated medium for Nocive Brewers bacteria Agar broth base modified - Condalab 500 g N/A - Cat. No. Description Pack. € 777337 Dehydrated medium for osmophilic agar - Condalab 500 g N/A - Cat. No. Description Pack. € 777332 Dehydrated medium for nutrient broth - Condalab 500 g N/A - Cat. No. Description Pack. € 777328 Dehydrated medium for nutrient agar ISO 6579 / ISO 10273 - Condalab 500 g N/A - Medium for the cultivation of non-demanding germs in water and clinical samples. hStorage: 2 - 25 °C hIncubation: 35 ± 2 °C for 18 - 24 h Medium recommended by ISO 21528 for the confirmation of Enterobacteriaceae. hStorage: 2 - 25 °C (Dehydrated base and ready-to-use medium) hIncubation: 37 ± 1 °C for 24 ± 2 h Recommended for the enumeration of yeasts and moulds in milk, dairy products and food. hStorage: 2 - 25 °C (Dehydrated base and ready-to-use medium) hIncubation: 20 - 25 °C for 5 - 7 days Supplement For 500 ml of medium. hAdd 50 mg of oxytetracycline to 5 ml of sterile distilled water Cat. No. Description Units/ box €/ box 777791 Oxytetracycline - 50 mg bottle - Condalab 10 N/A - For the detection of osmophilic yeasts in foods hStorage: 2 - 25 °C hIncubation: 20 ± 20 °C for 48 - 72 h Ingredient used as a selective inhibitor in culture media such as BLBVB hStorage: 2 - 25 °C Cat. No. Description Pack. € 777448 Ox bile, bacteriological - Condalab 500 g N/A - 778405 Ox bile, bacteriological - Condalab 25 kg N/A - O.G.A. (Oxytetracycline Glucose Agar) (O.G.Y.E.)

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