Molecular Biology

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MOLECULAR BIOLOGY Filter tips and gel tips can be found in the chapter TIPS- MICROPIPETTES TIP An extraordinary adventure • Bead homogenisers 130 to 134 • Grinder/Homogeniser 134 and 135 • Dispersion grinders 136 to 138 • Purification Robotic Workstation 138 • Deepwell Plates for machines 139 • Fluorometer 140 • Thermal cyclers 140 to 148 • Electrophoresis: cuvettes, generators, gels, accessories 149 to 173 • Electroporation 173 • Ultrafiltration, centrifugal filtration, protein purification, concentrators, SARTORIUS Vivaflow filtration, MERCK MILLIPORE Multiscreen 174 to 181 • Filtering tubes 182 and 183

MOLECULAR BIOLOGY An extraordinary adventure • Decontamination of surfaces and instruments 186 • Collection, transport and storage of nucleic acid samples 187 and 188 • Nucleic acid electrophoresis Agarose, 189 to 191 Pre-cast gels, 192 Buffers, 192 and 193 Gel cutting accessories 193 and 194 • Blotting, hybridization and revelation Membranes and blotting papers for nucleic acids, 195 and 196 Membranes and blotting papers for proteins, 197 to 199 Revelation, 200 to 203 Accessories 204 to 207 • Reagents and chemicals for Molecular Biology and Biochemistry grades 207 • Alternatives to alkylphenol ethoxylate detergents 208 • PCR Microtubes 209 to 219 • PCR Plates 220 to 234 • PCR Films 235 to 240

Molecular Biology 130 Bead Homogenisers h4 tubes of 0.5 ml, 1.5 ml or 2 ml, or 1 tube of 7 ml can be homogenised simultaneously hSupplied with 0.5/1.5/2 ml tube holder and 7 ml tube holder hApplications: lysis of human tissues, animal tissues, plants and foods, micro-organisms Range of volumes 25 µl - 7 ml Speed (g) 30 - 150 Timer 1 s to 5 min Dimensions W x D x H (mm) 25.4 x 21.5 x 29.2 Weight (kg): 7.48 Power supply 100-230 V / 50 - 60 Hz Cat. No. 078160 € N/A - OMNI Mini Bead Ruptor 4 Bead Homogeniser

Molecular Biology 131 All prices are indicative and exclude VAT. Visit www.dulis.nl Bead Homogenisers Pre-filled tubes for Omni Bead Ruptor Elite and Bead Ruptor 4 homogenisers hPolypropylene hRNase, DNase free (except 7 ml tubes) Pre-filled tubes and homogenising beads for Omni Bead Ruptor Elite and Bead Ruptor 4 homogenisers Tube volume (ml) Beads Ø beads (mm) Applications Units/ box Cat. No. €/ box 0.5 Garnet 0.15 Small volume of microorganisms, lysis 50 078101 N/A - Carbide 0.25 RNA extraction, microorganisms, homogenisation 50 078103 N/A - Ceramic 1.4 Soft tissues, homogenisation 50 078104 N/A - 1.5 Metal 2.38 Hard tissues, mix 50 078176 N/A - Glass 0.5 Hard microorganisms, lysis 50 078177 N/A - Ceramic 1.4 Soft tissues, homogenisation 50 078178 N/A - Ceramic 2.8 Hard tissues, homogenisation 50 078179 N/A - 2 Ceramic 0.1 Micro-organisms, lysis 50 078220 N/A - Ceramic 0.1 Micro-organisms, lysis 50 078221* N/A - Ceramic 0.5 Hard microorganisms, lysis 50 078222 N/A - Ceramic 0.5 Hard microorganisms, lysis 50 078223* N/A - Ceramic 1.4 Soft tissues, homogenisation 50 078105 N/A - Ceramic 1.4 Soft tissues, homogenisation 50 078142* N/A - Ceramic 2.8 Hard tissues, homogenisation 50 078106** N/A - Ceramic 2.8 Hard tissues, homogenisation 50 078141* N/A - Ceramic 1.4 y 2.8 Hard tissues, homogenisation 50 078224 N/A - Metal 2.38 Hard tissues, grinding 50 078098** N/A - Metal 2.38 Hard tissues, grinding 50 078143* N/A - Metal 6 Hard tissues, grinding 50 078225** N/A - Glass 0.5 Hard microorganisms, lysis 50 078100 N/A - Glass 0.5 Hard microorganisms, lysis 50 078144* N/A - Glass 0.1 Micro-organisms, lysis 50 078099 N/A - Garnet 0.7 Hard and fibrous tissues, homogenisation 50 078102 N/A - 7 Metal 2.38 Hard tissues, grinding 50 078180 N/A - Ceramic 1.4 Soft tissues, mix 50 078181 N/A - Ceramic 2.8 Hard tissues, mix 50 078182 N/A - Glass 0.5 Micro-organisms, lysis 50 078234 N/A - 15 Ceramic 2.8 Hard tissues, homogenisation 50 078201 N/A - 30 Metal 2.4 Hard tissues, mix 50 078202 N/A - Metal 2.4 and ceramic cylinder 6.35 Hard tissues, mix 50 078203 N/A - Ceramic 1.4 and ceramic cylinder 6.35 Soft tissues, homogenisation 50 078204 N/A - Ceramic 1.4 Soft tissues, homogenisation 50 078205 N/A - Ceramic 2.8 Hard tissues, homogenisation 50 078206 N/A - Ceramic 2.8 and ceramic cylinder 6.35 Hard tissues, homogenisation 50 078207 N/A - 50 Ceramic 2.8 Hard tissues, homogenisation 50 078194 N/A - Tube volume (ml) Units/ box Cat. No. €/ box 0.5 500 078186* N/A - 1.5 500 078183 N/A - 1000 078184 N/A - 2 500 078115* N/A - 500 078114B N/A - 1000 078185* N/A - 1000 078209** N/A - 7 500 078188 N/A - 1000 078124 N/A - 15 100 078231 N/A - 500 078198 N/A - 30 100 078232 N/A - 500 078199 N/A - 50 100 078200 N/A - Single tubes Homogenisation tube and beads For lysis of all cell types and extractions of nucleic acids, proteins and enzymes, etc. h1.4 and 2.8 mm ceramic beads for soft animal, plant and human tissues: brain, liver, skin, leaves, gonad, muscles h2.38 mm metal beads for all types of hard tissue: hair, teeth, bones, seeds, corn, rice, nails, etc. h1 and 0.5 mm glass beads for microorganisms: spores, moulds, yeast, escherischia coli bacteria , ... h0.15 mm and 0.7 mm garnet beads for soil, clay and sediment samples h0.15 mm and 0.25 mm carbide beads for microbiological RNA and soil sample DNase RNase * Microbial DNA free ** Reinforced wall tube *Reinforced polypropylene **Low binding

Molecular Biology 132 hFor bead milling of samples from 25 to 150 ml hUp to 384 samples processed by grinding simultaneously (4 Deepwell 1 ml 96-well plates) hCompatible with microplates, Deepwell, 0.5/1.5 and 2 ml tubes hTo be completed with the accessories of your choice (microplate holder, rack for 24 microtubes, stainless steel milling jar of 25 or 50 ml) hBeadless shaking up to 576 samples (6 x 96-well microplates) hAdjustable stirring frequency (3 to 30 Hz) hTime adjustable from 5 seconds to 99 minutes Range of volumes ‹ 25 µl to 50 ml Stirring frequency 3 to 30 Hz Stirring time 5 sec to 99 min. Noise level (dB) ‹ 72 Dimensions W x D x H (mm) 38.1 x 43.18 x 22.86 Weight (kg): 42 Cat. No. 078162 € N/A - Accessories Cat. No. Description Units/ box €/ box 078163 Microplate holder (1 Deepwell 2 ml or 2 Deepwell 1 ml or 3 standard plates) 2 N/A - 078164 Rack for 24 microtubes 1.5 or 2 ml or 24 screw tubes 0.5 or 2 ml 2 N/A - 078165 Stainless steel Milling Jar 25 ml* 2 N/A - 078166 Stainless steel Milling Jar 50 ml** 2 N/A - 078167 Stainless steel bead 10 mm 10 N/A - 078168 Stainless steel bead 25 mm 5 N/A - 078107 Bag of metal beads 2.38 mm, 500 grams 1 N/A - 078172 Bag of stainless steel beads 5 mm, 500 grams 1 N/A - 078173B Deepwell SBS 96-square-well plate, 2 ml 10 N/A - 078174B Sealing mat for Deepwell plate 10 N/A - 078175 Pack of 5 x 2 ml Deepwell 96-square-well plates with 1 x 5 mm stainless steel bead per well and 10 sealing mats 1 pack N/A - 078226 Pack of 2 x 2 ml Deepwell 96-square-well plates with 1 x 0.1 mm ceramic bead per well and sealing mat 1 pack N/A - 078227 Pack of 10 x 2 ml Deepwell 96-square-well plates with 1 x 0.1 mm ceramic bead per well and sealing mat 1 pack N/A - 078228 Pack of 10 x 2 ml Deepwell 96-square-well plates with 1 x 1.4 mm ceramic bead per well and sealing mat 1 pack N/A - 078229 Pack of 2 x 2 ml Deepwell 96-square-well plates with 1 x 1.4 mm ceramic bead per well and sealing mat 1 pack N/A - *Operates with a 10 mm stainless steel bead, sample volume from 4 to 10 ml. **Works with a 25 mm stainless steel bead, sample volume from 8 to 20 ml. OMNI Bead Ruptor 96 bead homogeniser 078163 078164 078166 Bead Homogenisers hCompatible with microtubes: 1.5 or 2 ml (0 - 300 µl samples), 5 ml tubes (0.1 - 1 ml samples) or 50 ml tubes (0.1 - 3.5 ml samples) depending on model hAdjustable homogenisation speed h“4 °C cooling”System: maintains samples at 4 °C with the addition of dry ice (“gold” versions) hCE certification hSupplied with starter kit: bag of different types of beads, scoops and suitable tubes hGold" and "50-DX" versions with digital display and recordable programmes hWarranty: 2 years Bullet Blender Bead Homogenisers Model Storm Pro BT24M Gold BB24-AU 5E Pro BT5E 5 Gold BB5E-AU 50-DX BB50-DX 50 Gold BB50-AU Capacity 24 x 1.5 and 2 ml tubes 12 x 5 ml tubes 8 x 50 ml tubes Air cooling √ √ √ √ √ √ 4 °C cooling - √ - √ - √ Compatible with RINO Screw-cap tubes √ √ - - - - Digital display √ √ √ √ √ √ Operating temperature ( °C) 4 to 40 (non-condensing) Dimensions W x D x H (mm) 34.3 x 25.4 x 22.9 46 x 35 x 38 34.3 x 25.4 x 22.9 46 x 35 x 38 38 x 35 x 38 46 x 35 x 38 Weight (kg): 5 13.6 5 13.6 13.6 13.6 Cat. No. 152081 152047 152082 152049 152044 152050 € N/A - N/A - N/A - N/A - N/A - N/A - Model BB50-DX Gold models

Molecular Biology 133 All prices are indicative and exclude VAT. Visit www.dulis.nl Bead Homogenisers Pre-filled tubes Colour code Applications Bead Ø (mm) Tube volume (ml) Tube type RNase free Units/ box Cat. No. €/ box Green Soft tissues (muscles) Stainless steel 50 µl of 0.5 mm beads 50 µl of 0.9 - 2 mm beads 1.5 RINO No 50 152093 N/A - Yes 50 152094 N/A - No 250 152095 N/A - Yes 250 152096 N/A - 1.5 Eppendorf safe-lock No 50 152089 N/A - Yes 50 152090 N/A - No 250 152091 N/A - Yes 250 152092 N/A - 5 No 20 152085 N/A - Yes 20 152086 N/A - No 100 152087 N/A - Yes 100 152088 N/A - Blue Hard samples (bones, tumours, hard-shelled insects) Stainless steel 100 µl of 0.9 - 2 mm beads and 5 x 3.2 mm beads 1.5 RINO No 50 555668 N/A - Yes 50 555669 N/A - No 250 555670 N/A - Yes 250 555671 N/A - 1.5 Eppendorf safe-lock No 50 152062 N/A - Yes 50 152063 N/A - No 250 152064 N/A - Yes 250 152065 N/A - 5 No 20 555710 N/A - Yes 20 555711 N/A - No 100 555712 N/A - Yes 100 555713 N/A - Pink Soft and small samples (liver) Zirconium oxide 50 µl of 0.5 mm beads and 50 µl of 1 mm beads 1.5 RINO No 50 555726 N/A - Yes 50 555703 N/A - No 250 555727 N/A - Yes 250 555702 N/A - 1.5 Eppendorf safe-lock No 50 555722 N/A - Yes 50 555723 N/A - No 250 555724 N/A - Yes 250 555725 N/A - 5 No 20 555718 N/A - Yes 20 555719 N/A - No 100 555720 N/A - Yes 100 555721 N/A - Red Soft and large samples Zirconium oxide 100 µl of 0.5 mm beads and 100 µl of 1 mm beads 1.5 RINO No 50 555707 N/A - Yes 50 555705 N/A - No 250 555708 N/A - Yes 250 555704 N/A - 1.5 Eppendorf safe-lock No 50 555732 N/A - Yes 50 555733 N/A - No 250 152074 N/A - Yes 250 555734 N/A - 5 No 20 555728 N/A - Yes 20 555729 N/A - No 100 555730 N/A - Yes 100 555731 N/A - Yellow Bacterial lysis Ceramic 200 µl of 0.15 mm beads 1.5 RINO No 50 555739 N/A - Yes 50 555740 N/A - No 250 555741 N/A - Yes 250 555742 N/A - 1.5 Eppendorf safe-lock No 50 555735 N/A - Yes 50 555736 N/A - No 250 555737 N/A - Yes 250 555738 N/A - Orange Bacterial extraction, generation of cell suspensions from tissue samples Stainless steel 5 x 3.2 mm beads 1.5 RINO Yes 50 555715 N/A - Yes 250 555717 N/A - 1.5 Eppendorf safe-lock Yes 50 555714 N/A - Yes 250 555716 N/A - Cat.No. Description Units/ bag €/ bag 152012 Measuring spoons 1.2 ml, single pack, sterile 200 N/A - 152013 20 N/A - 152010 Spatulas 50 µl, single pack, DNase/RNase free, PCR clean, antistatic, sterile 50 N/A - Single tubes Reinforced tube for vigorous homogenisation. Screw cap with gasket to prevent sample loss. Recommended for use with metal grinding beads Ref. Description Units/ box €/ box 152051 1.5 ml RINO microtubes, sterile 500 N/A - Microtubes 1.5 ml reinforced wall Ref. Description Units/ box €/ box 033508 Eppendorf Safe-Lock Microtubes 1.5 ml, PCR clean 1000 N/A - 033509 Eppendorf Safe-Lock Microtubes 2 ml, PCR clean 1000 N/A - Safe-Lock microtubes Ref. Description Units/ box €/ box 933008 Eppendorf tubes 5 ml DNase/RNase free, sterile 200 N/A - Tubes Accessories

Molecular Biology 134 Bead homogenisers / Homogenising mills For homogenisation of samples that are too resistant to vortexing, lysis of all types of cells. Economical alternative to other homogenisers. hFor simultaneous homogenisation of up to 3 (BeadbugTM) unskirted 2 ml tubes with screw cap or up to 6 (BeadbugTM 6) x 2 ml tubes or 2 (BeadbugTM 6) x 5 ml* tubes hGlass beads and stainless steel beads (acid washed) available in pre-filled tubes and in bulk (to be purchased separately) hTriple Pure nuclease-free zirconium beads (molecular biology grade) hWarranty: 2 years CatNo. Description Units/ box €/ box 062832 Adapter for 2 x 5 ml tubes for BeadbugTM 6 1 N/A - 062833 5 ml tubes with unmounted cap for homogeniser 500 N/A - Model BeadbugTM Speed 2700 to 4000 rpm Capacity Up to 3 x 2 ml tubes (screw cap, non skirted) Timer 3 sec to 3 minutes Dim. L x W x H / Weight 17 x 21 x 13.5 cm / 2.2 kg Voltage 230 V Cat. No. 062505 € N/A - Model BeadbugTM 6 Speed range (m/s) 4 to 7 (in increments of 0.05 m/s) Capacity 6 x 2 ml tubes or 2 x 5 ml tubes* Cycle time (sec) 1 to 90 Pause between cycles (sec) 0 to 90 (in 1 sec increments) Max. number of cycles 10 Noise level (dB) ‹ 65 Dimensions (cm) 22 x 35.5 x 20.5 Power supply 100 to 240 V, 50-60 Hz Weight (kg): 9 Cat. No. 062831 Voltage 100 to 240V Cat. No. 062831 € N/A - BeadBugTM and BeadBugTM 6 Bead Homogenisers *Requires adapter sold separately Accessories hSturdy and user-friendly disrupter with adjustable grinding time and force hThe tube is shaken in a figure of 8 pattern for better percussion and bead distribution hProcesses 24 samples of up to 400 mg each (0.5 /1.5 /2 ml screw tube) hAdapter for 24 tubes included hAdapter for 8 x 7 ml tubes available as an option hGrinding of all types of samples: bacteria, yeast, tissue, hair, bone, tooth.. hVarious tubes available: reinforced polypropylene, disposable stainless steel Mini-beadbeater 24 Grinder/homogeniser Timer 0 to 5 minutes Oscillation speed 2000 to 3450 strokes/min Oscillation amplitude (cm) 2.22 Dimensions (W x D x H) (cm) 45.72 x 25.4 x 30.48 Power supply 115-230 V, 50/60 Hz Cat. No. 918007 € N/A - Cat.No. Description Units/ box €/ box 918012 XXTUFF 2 ml Tube 500 N/A - 918009 Stainless steel 1.8 ml tube 100 N/A - 918021 Silicone cap for stainless steel tube 100 N/A - Accessories h XXTuff reinforced polypropylene tube hSame dimensions as a 2 ml microtube hReinforced tube allowing the use of steel beads hScrew cap with seal h Stainless steel Tube hSame external dimensions as a 2 ml microtube hImproves heat exchange and resistance hIdeal for applications where polypropylene could contaminate the sample hRecommended for grinding under nitrogen hSilicone caps are required for grinding dry or frozen samples using steel beads (sold separately)

Molecular Biology 135 All prices are indicative and exclude VAT. Visit www.dulis.nl Homogenising mills hStainless steel: for plant tissue hCarbide: for microbiological RNA and soil samples hCeramic: for plant, animal and soft human tissue hGarnet: for hard samples, not suitable for soil samples hMetal: for all types of hard tissues hZirconium oxide: for high viscosity samples hZirconium silicate: for spores and most tissues hGlass: for micro-organisms hZirconium: for hard tissue hSterile (except 50 ml tubes) hUFO beads: special shape for grinding fibrous or elastic samples Beads for grinder/homogeniser Bead tubes Bead volume (ml) Material Ø (mm) Units/ box Cat. No. €/ box 4 Stainless steel 0.2 1 152033 N/A - 0.5 1 152035 N/A - 0.9 to 2 1 152037 N/A - 1.6 1 152052 N/A - 10 3.2 1 152053 N/A - 4.8 1 152054 N/A - 6 1 152056 N/A - 10 Stainless steel - UFO bead 3.5 1 152058 N/A - 50 1 152057* N/A - 10 5.6 1 152060 N/A - 50 1 152059* N/A - 4 Zirconium oxide 0.15 1 152025 N/A - 0.5 1 152027 N/A - 1 1 152029 N/A - 2 1 152031 N/A - 4 Glass 0.1 1 152017 N/A - 0.5 1 152019 N/A - Triple Pure bead bottles Material Ø (mm) Weight (g) Cat. No. € Zirconium 0.1 250 062809 N/A - 0.5 250 062776 N/A - 1 250 062815 N/A - 1.5 250 062816 N/A - 3 300 062773 N/A - hMolecular biology beads, certified free of RNAse, DNAse, proteases and nucleic acids * Non sterile. Bags of beads Material Ø (mm) Weight (g) Cat. No. € Stainless steel 0.2 450 152032 N/A - 0.5 450 152034 N/A - 0.9 to 2 450 152036 N/A - 1.6 450 152038 N/A - 2.3 450 555743 N/A - 3.2 450 152039 N/A - 4.8 450 152040 N/A - 6 450 152055 N/A - Ceramic 1.4 325 078112 N/A - 2.8 325 078113 N/A - 6.5 325 078219 N/A - Garnet 0.15 500 078110 N/A - 0.7 500 078111 N/A - Metal 2.38 500 078107 N/A - Zirconium oxide 0.15 450 152024 N/A - 0.5 450 152026B N/A - 1 450 152028 N/A - 2 450 152030 N/A - 2.5 450 152080 N/A - Zirconium silicate 0.1 450 918006 N/A - 0.5 450 918000 N/A - 0.5 450 555744 N/A - 1 450 152022 N/A - 450 918015 N/A - Material Ø (mm) Weight (g) Cat. No. € Glass 0.1 375 079052 N/A - 400 078108 N/A - 450 152016 N/A - 450 918004 N/A - 0.5 375 079053 N/A - 400 078109 N/A - 450 152018 N/A - 450 918014 N/A - 1 450 152020 N/A - 1 1000 068499 N/A - 2 1000 068500 N/A - 3 1000 068501 N/A - 4 1000 068502 N/A - 5 1000 068503 N/A - 6 1000 068504 N/A - 8 1000 068505 N/A - 10 1000 068555 N/A - Zirconium 2 450 918016 N/A -

Molecular Biology 136 Dispersion mill hPower: 125 W hEconomical for samples from 0.25 to 30 ml hVariable speed motor from 5 000 to 35 000 rpm hSupplied with a 7 mm x 85 mm C189 dispersion rod hMost samples are dispersed in less than a minute hEasy to clean and dismantle hPortable and lightweight hTo be handled by hand or mounted on a stand hCSA, UL and CE C196 standards hNoise below 72 dB h1 year warranty for the motor TissueMaster-125 Omni Homogeniser hTo be held in the hand or on a stand hFor samples from 0.05 to 100 ml hLightweight, ergonomic hSupplied with probe/dispersion rod assembly tool and manual h125 W motor hMounting of EZ connect stainless steel probes with 1/4 revolution bayonet hNoise limited to 72 dB hVariable speed from 5 000 to 35 000 rpm hCE and CSA certified TH pack with TH 220 V homogeniser motor , 3 EZ-Connect rods for fine grinding (5 x 75 mm flat bottom (Cat No. 078007), 7 x 115 mm saw teeth (Cat. No. 078148) and 10 x 115 mm saw teeth (Cat. No.. 078150), nut and stand. hFor samples from 200 µl to 120 ml hTo be held by hand or on a stand hVariable speed from 8 000 to 32 000 rpm hMax. noise level 68 dB h40 W motor hSupplied with assembly tool, stand clamp, manual and starter kit including 12 plastic dispersion rods, 6 for soft tissue and 6 for hard or frozen tissue hDispersion rod: - Ø: 7 x 110 mm - Recommended working volume: 0.25 A 30 ml TH Omni Homogeniser THQ Omni Homogeniser CatNo. Description € 078146 GLH-850 homogeniser complete with 3 stainless steel rods (10, 20 and 30 mm) N/A - 078147 GLH-850 Single motor homogeniser N/A - Cat. No. Description € 078053 TissueMaster-125 Homogeniser withrod 7 x 85 mm N/A - Cat. No. Description € 078000 Full TH pack N/A - 078001 Omni Tissue Homogeniser only N/A - 078002 Stand N/A - 078003 Rod with tightening nut N/A - Cat. No. Description € 078211 THQ homogeniser N/A - 078002 Stand N/A - Omni GLH-850 Homogeniser hFor samples from 1.5 ml to 5 l h850 W motor, 2 year warranty hVariable speed: 5000 to 25000 rpm hNoise level: < 72 dB hTimer up to 9 min 59 sec hOn / off button hBase included hCE certified

Molecular Biology 137 All prices are indicative and exclude VAT. Visit www.dulis.nl Dispersion mill Cat. No. Description € 078026 Dismantling tools N/A - Cat. No. Dimensions Ø x h (mm) Volume (ml) Bottom Type of grinding € 078007 5 x 75 0.02 - 5 Flat Fine N/A - 078008 5 x 75 0.02 - 5 Flat Coarse N/A - 078148 7 x 115 0.25 - 30 Serrated Fine N/A - 078149 7 x 115 0.25 - 30 Serrated Coarse N/A - 078150 10 x 115 1.5 - 100 Serrated Fine N/A - 078151 10 x 115 1.5 - 100 Serrated Coarse N/A - Cat. No. Dimensions Ø x h (mm) Volume (ml) Bottom Type of grinding € 078021 20 x 195 100 - 2000 Serrated Fine N/A - 078022 20 x 195 100 - 2000 Serrated Coarse N/A - 078152 30 x 195 200 - 5000 Crenellated Fine N/A - 078153 30 x 195 200 - 5000 Crenellated Coarse N/A - Rods for Omni TH and GLH 850 homogenisers Rods for Omni GLH 850 homogeniser only Accessoire Omni stainless steel dispersion rods with rotor stator rod Cat. No. Dimensions ø x H (mm) Volume (ml) Bottom Units/ box €/ box 078212 5 x 75 0.2 - 5 Serrated 1 N/A - 078213 5 x 75 0.2 - 5 6 N/A - 078214 7 x 100 0.25 - 30 1 N/A - 078215 7 x 100 0.25 - 30 6 N/A - 078216 10 x 110 1.5 - 100 1 N/A - 078217 10 x 110 1.5 - 100 6 N/A - Rods for THQ Omni homogeniser 078007 078149 078022 078008 078151 078152 078148 078021 078153 The photos on the right are not to scale with each other. 078212 078213 078214 078215 078216 078217 hReusable and autoclavable up to 7 times hTo eliminate the risk of cross-contamination between samples hIdeal for PCR, DNA or RNA blotting analysis hThe clear plastic rod tube shows that there is no sample left hCan be cleaned, disinfected and sterilised for repeated use hTwo-piece construction for easy disassembly and cleaning h80% less protein residue compared to a stainless steel tool hCompatible with all Omni homogenisers with EZ connection (GLH, TGH, THQ) hAvailable in sterile version: avoids cross-contamination and transfer between samples hDiameter 7 x 110 mm hRecommended workload: 0.25 ml to 30 ml Two models: • rods for standard dispersion of liquid mixtures, soft tissues or tissues requiring gentle homogenisation; • rods for the disintegration of hard or frozen tissues. Dispersion rods In boxes 25 50 100 500 Omni TipTM standard 110 mm dispersion rods Cat. No. 078031 078032 078033 078034 € N/A - N/A - N/A - N/A - Omni TipTM Dispersion Rods for hard or frozen tissues 110 mm Cat. No. 078035 078036 078037 078038 € N/A - N/A - N/A - N/A - Omni TipTM Dispersion Rods for hard or frozen tissues 110 mm sterile Cat. No. 078039 - - - € N/A - - - - Cat. No. 078042 Adapter for homogenisers Omni EZC 1 N/A - and Fisher PowerGen Description €/ box Units/ box Adapter Plastic dispersion rods: Omni TipTM 078042

Molecular Biology 138 Cat. No. € PCR Manual Adapter Cleaning Kit Stainless steelrod ø x H (mm) 12 Omni TipTM ø x H (mm) Homogeniser Omni TH 078043 Yes 7 x 110 No No Yes Yes N/A - 078045 Yes 7 x 110 5 x 75 Yes Yes Yes N/A - 078046 Yes 7 x 110 7 x 115 Yes Yes Yes N/A - 078047 Yes 7 x 110 10 x 115 Yes Yes Yes N/A - Omni PCR soft tissue kit, kit contents: Cat.No. € PCR Manual Adap- ter Steelrod Stainless steel ø x H (mm) 12 Omni TipTM ø x H (mm) Homogeniser Omni TH 078051 Yes 7 x 110 5 x 75 Yes Yes N/A - Omni PCR hard tissue kit, kit contents Cat. No. 078026 Dismanting tools N/A - 078002 Stand for Omni TH N/A - 078003 Stem with mounting nut N/A - Description € Accessories hPlastic dispersion rods, autoclavable hFor the complete disintegration of cell lysates hGood disintegration of membranes hSteel dispersion rods: for samples that are not susceptible to contamination hPlastic rods, single or limited use to avoid contamination hFlat bottom for soft tissues and serrated tool for fibrous tissues Each kit includes a TH homogeniser with PCR instructions, Omni TipTM adapter, stainless steel dispersion rod, storage case. Two models: for soft tissue or for frozen tissue. Omni TH tissue homogenisation kit for PCR Sterilisation of Omnitips RODS Recommended • Autoclave up to 121 °C • Formaldehyde(g) • Ethylene oxide • Gamma irradiation up to 5 MRAD • Glutaraldehyde (2%) • Nitrogen oxide Not Recommended • UV irradiation Biological disinfection Recommended • Hypochlorite bleach (5%) • Formaldehyde 40% • 70% alcohol Not Recommended • Phenolic derivatives • Long-term exposure to Guanidine derivatives • Dichloralphenazone Decontamination after using radioactive products Recommended • Water / Ethanol / SDS • Cleaning solutions: "radiacwash" • or "count-off Cleaning of the Omnitips Plastic ROD Adapter Recommended • Cleaning with soapy water • Hot water and brief rinse with 1% SDS, then distilled water treated with diethyl procarbonate (DEPC) Dispersion mill / Purification machine Magnet End for tips Wells Plates compatible with Kingfisher 96 Flex Magnetic Particle Processors. For storage, centrifugation, preparation and purification of samples. hMade of virgin polypropylene hSlightly raised well edges to avoid crosscontamination hHigh stability during centrifugation hAlphanumeric marking hSquare wells with cylindrical bottom for better mixing hU-shaped bottom Cat.No. Description Vol. tot. (ml) Working Vol. (µl) Units/ box €/ box 023131B Riplate® magnetic 96-well plates 0.2 20 - 100 60 N/A - 071474 Riplate® magnetic 96-well plates * 2.0 20 - 1000 20 N/A - 071475 Riplate® magnetic 96 comb* - - 60 N/A - 023131B 071474 Riplate® magnetic 96-well plates *Compatible with KingFishet Flex and Presto robots

Molecular Biology 139 All prices are indicative and exclude VAT. Visit www.dulis.nl Deepwell Plates for machines hUsed in DNA/RNA extraction kits and DNA extraction machines hCompatible with MagnaPure 96 hMade of low binding polypropylene hOperating temperature: -80 °C to +100 °C hLaser engraved barcode h Deepwell 96-well for extraction steps hMarking of volumes 0.5 ml, 1 ml, 1.5 ml and 2 ml on 2 longitudinal sides hHigh walls to limit cross-contamination MagnaPure 96 Roche Plates for machine Pyrogenic DNase RNase Human DNA RNA 96-well V-bottom plate for collection of purified nucleic acid eluates Cat. No. Description Pack. unit Units/ box €/ box 038342 Deepwell, 96-well 5 30 N/A - 038343 96-well V-bottom plate 20 20 N/A - 038342 038343 hTransparent virgin polypropylene hWell 1 ml round, V bottom hAlphanumeric marking hSuitable for separation protocols using magnetic beads hUltra-flat hManufacturing ISO 7 and 8 rooms hResistant to DMSO, phenol, chloroform hSBS format hOperating temperature: -80 °C to 120 °C hAutoclavable Cat. No. Description Units/ box €/ box 448027 Deepwell 96-well plate 50 N/A - DNase RNase Human DNA Endotoxins Deepwell 96-well plate for magnetic bead separation hMade of virgin polypropylene hManufactured in clean room hLow affinity for biomolecule hChemical and thermal resistance (-196 °C to +120 °C) hAutoclavable hAlphanumeric marking hKingFisher™ Flex, Duo Prime, and Presto compatible Cat. No. Volume (ml) Working volume (µl) Bottom Pack. unit Units/ box €/ box 058148 2.2 50 to 100 V 5 10 N/A - Deepwell 96 wells 2.2 ml for KingFisher DNase RNase Human DNA

Molecular Biology 140 Cat. No. Dimensions (mm) Packaging 036445 100 x 150 25 notebooks of 25 sheets N/A - 036531 200 x 300 100 N/A - 036532 460 x 570 500 N/A - € High quality paper for cleaning optical surfaces and lenses made of plastic glass or quartz. Does not contain any chemical agents, silicone or special additives. hVery soft, does not scratch hVery pure, free of additives hHighly absorbent for grease and moisture removal hIn Japanese paper hPaper for wiping lenses hLint free hSold in packs of 25 sheets Cat. No. 068885 95 x 135 25 bags of 25 sheets N/A - 068886 190 x 135 500 sheets N/A - 068887 190 x 270 500 sheets N/A - 068888 380 x 540 500 sheets N/A - Dimensions (mm) Packaging €/ box ECO lens cleaning wipes Cat. No. Format (mm) Units/ box €/ box 074042 95 x 135 25 x 25 N/A - 074089 100 x 150 625 N/A - 074090 200 x 300 100 N/A - 074091 460 x 570 500 N/A - Fluorometer / Thermocyclers Optical paper Optical paper for microscopes The QuantGene 9600 is a 96-well block real-time PCR thermal cycler. hReading: 5 fluorescence channels for multiplexing (green, yellow, orange, red, infrared); possibility to configure a 6 th channel as desired hConsumables: 96-well 0.2 ml PCR plates, 0.2 ml PCR individual tubes or strips of 8 hStand-alone: integrated large touch screen, running Windows 10 hTemperature range of the block: 4 - 105 °C hTemperature ramp: 6 °C/sec hLid temperature: 30 - 110 °C hWarranty: 27 months QuantGene 9600 Real-Time Thermocycler Dimensions W x D x H (mm) 38 x 40 x 38 Weight (kg): 20 Cat. No. 865083 € N/A - DMV-Bio quartz cuvettes for measuring microvolumes of nucleic acids and proteins. hFor measuring the absorbance of small volumes of nucleic acids and proteins with a conventional spectrophotometer hFor a beam height of 8.5 or 15 mm depending on the cuvette, compatible with most UV/visible spectrophotometers on the market hFor measuring 0.6 to 2.5 µl of sample depending on the model hValidation of the 260/280 nm ratio and absorbance value with solutions sold separately hProduction of a certificate for the accuracy of the optical path (± 2 µm) (optional when ordering, please consult us) Low volume cuvettes for DNA and proteins Optical path (mm) 0.125 0.2 0.5 Minimum sample volume (µl) 0.6 1 2.5 DNA detection limit (ng/µl) 7.1 3 1.2 Maximum DNA concentration (ng/µl) 12000 9000 3500 Reproducibility for 100 ng/µl DNA (ng) ±4 ±2 ±1 Reproducibility for 1000 ng/µl DNA (ng) ±7 ±6 ±4 Protein detection limit (mg/ml) 0.3 0.15 0.06 Maximum protein concentration (mg/ml) 100 Cat. No. Optical path (mm) Minimum sample volume (µl) Beam height (mm)* € 244500 0.5 2.5 8.5 N/A - 244501 0.5 2.5 15 N/A - 244502 0.2 1 8.5 N/A - 244503 0.2 1 15 N/A - 244504 0.125 0.6 8.5 N/A - 244505 0.125 0.6 15 N/A - * Check the beam height of your spectrophotometer. Accessories Cat. No. Description € 244512 260/280 nm ratio validation solution, 1.5 ml N/A - 244513 Optical path length and absorbance validation solution, 1.5 ml N/A -

Molecular Biology 141 All prices are indicative and exclude VAT. Visit www.dulis.nl Véronique VAN DEN BOSSCHE is ready to help you with all your questions: quotation requests, offers & prices. Do not hesitate to contact her at +32 81 94 66 00 or by email veronique.vandenbossche@dulis.be WELCOME TO DULIS CUSTOMER SERVICE & PURCHASING Véronique VAN DEN BOSSCHE, Customer Care Coordinator. SERVICE, RESPONSIVENESS AND AVAILABILITY ARE PART OF OUR DAILY PRIORITIES. TO FOLLOW UP YOUR ORDERS, PRODUCTS AVAILABILITY, DEADLINES, please contact your advisor by email: orders@dulis.be ANY QUESTIONS? NEED INFORMATION? VISIT OUR WEBSITE www.dulis.be I www.dulis.nl OR CONTACT US VIA info@dulis.be

Molecular Biology 142 Thermal cyclers Aluminium block Gold-plated silver block Temperature ( °C) -5 to +99.9 Homogeneity ( °C) ± 0.25 to 55 °C, ± 0.4 to 95 °C Heating rate ( °C/s) 3.5 5 Cooling rate ( °C/s) 3.2 5 De(In)crement temperature ( °C) 9.99 De(In)crement time (seconds) 99.99 Heated lid Automatic, up to 105 °C Number of programmes Up to 680 Dimensions (L x D x H )(cm) 20 x 37 x 16 Weight (kg) 7.8 Basic Gradient Gradient - 40 °C max ( ± 20 °C) Temperature ( °C) -5 to +99.9 Homogeneity ( °C) ± 0.25 from 20 °C to 72 °C, ± 0.4 to 95 °C Temperature setting increment ( °C) 0.01 De(In)crement temperature ( °C) 9.99 De(In)crement time (seconds) 99.99 Gradient - 40 °C max ( ± 20 °C) Number of programmes up to 680 Dimensions (L x D x H )(cm) 25.1 x 44.4 x 20.1/34.7 (lid closed/open) Weight (kg) 11.5 hAvailable in basic or gradient version, with 48-well aluminium or gold-plated silver block (included) hTFT touch screen h8-zone gradient depending on model hHeating and cooling rate up to 5 °C/second depending on the model hTime and temperature decrement or increment possible (up to ± 9.99 °C and ± 99.99 seconds) hAccess code protection for up to 64 users hLow energy consumption hGradient 20 °C max (± 10 °C)* hWarranty: 5 years Basic 48-well non-removable model Cat. No. 049974 049986 € N/A - N/A - 48-well non-removable gradient model Cat. No. 049975 049976 € N/A - N/A - Labcycler 48 basic or gradient Thermal cyclers hTFT touch screen and keypad hVarious interchangeable blocks available (to be ordered separately), triple block 3 x 21 wells for three independent programs, 48, 96 and 384 wells hTool-free block change with automatic block recognition hAluminium 96-well block for reactions up to 100 µl, ideal for high volume in vitro reactions hGradient of 8 to 24 zones depending on model and block hHeating and cooling rates up to 4.2 and 3.6 °C/second respectively, depending on model and block hGradient 40 °C max (± 20 °C)* hUp to 680 programmes can be stored in memory hData protection by access code, by group or individual, file or program hWarranty: 5 years Cat. No. Description € 049965 Upgrade service from a basic gradient model N/A - 049971 Lid for one 21-well block of the triple block N/A - Cat. No. Description Heating / cooling rate ( °C/s) Number of zones for gradient* Min/max reaction volume per well (µl) € 049964 48-well gold-plated silver block (0.5 ml) 4.2 / 3.6 8 20 / 200 N/A - 049963 96-well gold-plated silver block (0.2 ml) 4.2 / 3.6 12 10 / 80 N/A - 049962 384-well gold-plated silver block 4.2 / 3.6 24 3 / 20 N/A - 049970 Triple block 3 x 21 wells** 2.5 / 2.2 - 10 / 80 N/A - 049982 Aluminium block 96-well 100 µl, 0.2 ml 2.5 / 2.2 12 10 / 100 N/A - 049985 Block for in situ PCR 2.5 / 2.2 - - N/A - * only valid for the gradient labcycler *triple block is not gradient compatible, passive covers to prevent condensation to be ordered separately (ref.049971) Options Block for labcycler Basic or gradient Labcycler thermal cyclers 049975 Labcycler Cat. No. 049984, with 96-well block Cat. No. 049963 Thermal cycler labcycler without block Cat. No. 049984 049983 € N/A - N/A - * Only valid for the gradient labcycler.

Molecular Biology 143 All prices are indicative and exclude VAT. Visit www.dulis.nl Block temperature range 4 to 99.9 °C Gradient range 1 to 24.9 °C Gradient Temperature range 30 to 99 °C Lid temperature 35 °C to 120 °C or inactive Block Homogeneity ± 0.3 °C Block temperature Accuracy ± 0.3 °C Max. heating rate 5.5 °C / sec Max. cooling rate 3.3 °C / sec Adjustable temperature ramp 0.1 to 5.5 °C / sec Recordable programmes 500 Interface USB, WIFI (optional) Dim. W x D x H (mm) 245 x 415 x 225 Weight (kg) 9.5 Power supply 100 - 240 V, 50/60 Hz Max. power consumption 750 W Cat. No. 255200 € N/A - Block temperature range 4 to 99.9 °C Gradient range 1 to 24 °C Gradient Temperature range 30 to 99 °C Lid temperature 35 °C to 120 °C or inactive Block Homogeneity ± 0.5 °C Block temperature Accuracy ± 0.3 °C Max. heating rate 3 °C Max. cooling rate 2 °C Recordable programmes 200 Dim. W x D x H (mm) 245 x 415 x 225 Weight (kg) 9 Power supply 100 - 240 V, 50/60 Hz Max. power consumption 500 W Cat. No. 255201 € N/A - h96-well gradient block compatible with standard and low profile 0.2 ml PCR tubes and strips, skirted, semi-skirted and unskirted plates h7'' colour touch screen that can be operated with laboratory gloves hIntuitive control interface with icons and graphics hHeated lid hMaximum heating / cooling rate 5.5 / 3.3 °C per second hConfigurable temperature ramp: 0.1 to 5.5 °C /sec hTemperature accuracy and uniformity of ± 0.3 °C hTemperature gradient from 1 to 24.9 °C on 12 columns for PCR parameter optimisation h96-well gradient block compatible with standard and low profile 0.2 ml PCR tubes and strips, skirted, semi-skirted and unskirted plates h240 x 128 pixel monochrome display with intuitive interface hTouch button control hHeated lid (35 °C to 120 °C or inactive) hMaximum heating / cooling rate 3 / 2 °C, adjustable speed (slow or fast) hTemperature accuracy / uniformity of ± 0.3 / 0.5 °C hTemperature gradient on 12 columns for optimization of PCR parameters TurboCycler 2 gradient thermal cycler TurboCycler lite gradient thermal cycler Accessoire Cat. No. Description Units/ box €/ box 255204 Compression mat for use with low profile tubes, bars or plates 10 N/A - Accessoire Cat. No. Description Units/ box €/ box 255204 Compression mat for use with low profile tubes, bars or plates 10 N/A - Thermal cyclers

Molecular Biology 144 Thermal cyclers The MaxyGene II is a thermal cycler with a 96-well block consisting of 6 independent Peltier elements. Up to 6 different annealing temperatures can be programmed to optimise PCR conditions. hRamps: heating +5 °C/sec, cooling -3.5 °C/sec hTouchdown programming possible (time and/or temperature increment/decrement) hHeated lid with programmable temperature hConsumable: 1 x 96-well plate 0.2 ml, 12 x 8-tube strips 0.2 ml and 96 x PCR tubes 0.2 ml hConnectivity: USB and RS232 ports hFor standard PCR application hWarranty: 3 years Axygen MaxyGene II Thermal cycler Dimensions L x D x H (mm) 24 x 43 x 25 Weight (kg) 9 Cat. No. 935649 € N/A - hCompact hAdjustable heated lid hDisplay of graphics, 100 storable programs Block material Aluminium Sample capacity 1 x 24-well microplat or 24 x 0.2 ml tubes (or 3 strips of 8) Heating rate 5 °C/sec Cooling 4 °C/sec Temp. range 4 - 99.9 °C Temp. uniformity ± 0.2 °C Temp. accuracy ± 0.2 °C Lid 30 - 110 °C Gradients - Number of programmes 100 Display LCD screen Dimensions ( W x D x H) 297 x 212 x 200 mm Weight 3.2 kg Power 200 W Excl. VAT 865004 € N/A - GeneQ TC 24 Block material Aluminium alloy Sample capacity 1 x 96-well skirted microplate or 96 x 0.2 ml microtubes (or 12 strips of 8) Temperature range of the block 4 °C - 105 °C Heating or cooling rate 4 °C / sec Temperature uniformity ± 0.3 °C Temperature accuracy ± 0.1 °C , ± 0.2 °C to 90 °C Temperature control mode Block or tube Temperature range of the gradient 30 - 110 °C Gradients 1 - 30 °C Gradient block On 12 columns Number of programmes 250 (expandable via USB port) Display LCD touch screen W x D x H (mm) / Weight (kg) 335 x 260 x 270 / 10 Power (W) 600 Cat. No. 865031 € N/A - hWith 5.7" touch screen hSmall footprint hHeated lid with adjustable height hInterface for USB connection hNetwork function: one computer can control several LifeEco thermal cyclers h250 program memory, expandable via USB port Model Gene Q TC 24 Bioer Thermal Cycler LifeEco Bioer Gradient Thermocycler

Molecular Biology 145 All prices are indicative and exclude VAT. Visit www.dulis.nl Thermal cyclers hWith touch screen hHeated lid with adjustable height hAluminium alloy block with excellent conductivity hInterface for USB connection 2 models: • 865018: for 96-well plates or strips, supplied with block • 865019: supplied without block, to be completed with 5 blocks, for 96 or 384 well PCR microplate,for in situ hybridization, for 48 tubes Cat. No. Description € 865018 LifeTouch thermal cycler with 96-well block N/A - 865019 Thermocycler Genetouch without block N/A - 865020 Block for Genetouch Bi-Bloc for 48 x 0.2 ml microtubes N/A - 865021 Block for Genetouch Bi-Bloc for 30 x 0.5 ml microtubes and 48 x 0.2 ml microtubes N/A - 865022 Block for Genetouch for 384 well skirted plate N/A - 865023 Block for Genetouch for 96 x 0.2 ml in gradient N/A - 865024 Block for Genetouch for in situ hybridization N/A - 865018 LifeTouch and GeneTouch Bioer Thermal cyclers hTouchscreen: 26.4 cm hVery uniform temperature without edge effect hAdjustable heated lid hMultiple units can be controlled via PC hControl possible via Bluetooth hNickel-plated copper block hFor 96-well skirted plate or 96 x 0.2 ml microtubes or 12 strips of 8 tubes GeneMax Bioer thermal cycler 96-well skirted plates Capacity 96 x 0.2 ml Tubes 12 x 8-tube strip Temperature range ( °C ) 4.0 to 105 Heating rate ( °C ) Up to 5 °C/sec Cooling rate ( °C ) Up to 4 °C/sec T °C Uniformity ≤± 0.2 °C T °C Accuracy ≤± 0.1 °C (at 55 °C) ≤± 0.15 °C (at 72 °C and at 95 °C) Temperature control Block or tube Heating/cooling rate control 0.1 to 4 °C Memory ≥ 250 programs (internal memory) or limited to the USB stick capacity Maximum number of cycles 99 Change in elongation time with each cycle 0 to 9 min 59 sec Change in elongation temperature with each cycle 0.1 to 9.9 °C Gradient function Yes Minimum/maximum gradient per line 0.1 to 6 °C Lid temperature ( °C) 30 - 110 Lid height Automatic adjustment Communication interface RS232, LAN, Bluetooth Power supply 100-240V 50-60Hz, 600W Dimensions L x W x H(mm) 457 x 316 x 309 Weight (kg) 14 Cat. No. 865070 € N/A -

Molecular Biology 146 hCompact and easy to use hTouch screen, also works with gloves hBlock for 4 strips of 8 tubes or for 32 individual tubes hAdjustable and deactivatable lid heating Capacity 32 x 0.2 ml tubes Temperature range 0 °C - 99.9 °C Accuracy / Homogeneity ± 0.25 °C / ± 0.25 °C Heating rate 5 °C / s Cooling rate 4 °C / s Temperature range of the lid 30 °C - 112 °C Number of programmes 100 Voltage 100 - 240 V, 50 - 60 Hz Dimensions L x D x H (mm) 182 x 232 x 157 Weight (kg) 2.9 Cat. No. 062757 € N/A - Mini thermal cycler Number of cycles Unlimited Number of steps Unlimited Temperature range 25 - 99 °C Heating rate 5.5 °C Cooling rate 4 °C Accuracy / homogeneity ± 0.4 °C Power supply, power 100 - 240 V, 50/60 Hz, 120W Ext. Dimensions W x D x H (mm) 136 x 102 x 104 Weight (kg) 1 Cat. No. 255209 € N/A - h17.8 cm diagonal colour LCD touch screen, usable with gloves hThe 96-well version has a multi-format block, suitable for unskirted 96-well plates, as well as 0.2 ml tube strips and 0.2 and 0.5 ml tubes hIntuitive programming, parameterisation of the steps with graphic display hHeated lid with adjustable height and temperature hProgramme protection by access code hAluminium block hTemperature gradient from 1 to 30 °C h100 program memory, expandable via USB port hApplications: PCR, RT-PCR and incubation hVery compact device hBlock for 8 conventional 0.2 ml PCR tubes (2 x 4) hHeated lid, fixed temperature of 105 °C (preheats to 60 °C) hProgramming on USB stick via PC (software included) TC9639 Gradient Thermal Cyclers MiniTurbo thermal cycler Model Multi-format 96 wells 384 wells Capacity 96-well non skirted plates; 96 x 0.2 ml tubes 8 strips of 12 tubes 0.2 ml and 39 x 0.5 ml tubes 384-well plates (non skirted) Temperature range ( °C) 0 to 100 Heating rate ( °C) Up to 5 °C/second Cooling rate ( °C) Up to 4 °C/second T °C Uniformity ± 0.2 T °C Accuracy ± 0.2 °C T °C control Block or tube* Gradient T° range 30 - 105 °C Gradient T° differential 1 - 30 °C Setting of the heating cover T° 30 - 112 °C Communication interface USB, LAN and RS232 Power supply 75 to 275 V, 50-60 Hz Dimensions L x D x H (mm) 26 x 36 x 25 Weight (kg) 7.3 Cat. No. 062817 062818 € N/A - N/A - * simulation of the temperature inside the tube. Thermal cyclers

Molecular Biology 147 All prices are indicative and exclude VAT. Visit www.dulis.nl Thermal cyclers 1, 2 or 4 block PCR units. Compact. hASC system - Active sample cooling for more specific amplifications hProgram wizard: generate a quick and easy protocol based on sequence, matrix origin and amplicon size hGradient: on all instruments, on all blocks, allows to target the right hybridization temperature h96/384 wells: flexibility of flow rate for different applications hUSB: connectivity for transferring protocols between machines hAndroid control, 7" (AC1) or 10" (AC2, AC4) screen: simple and intuitive use Model 1 block (AC1) 2 blocks (AC2) 4 blocks (AC4) Format 1 block 2 blocks 4 blocks Block options 96 or 384 well 96 or 384 or 1 x 96 - 1 x 384 96 or 384 or 3 x 96 - 1 x 384 or 2 x 96 - 2 x 384 or 3 x 96 - 3 x 384 Maximum temperature rise 3.4 °C/sec Temperature range 10 °C to 100 °C (4 °C storage temp.) Block uniformity at 55 °C ± 0.3 °C Temperature accuracy at 55 °C ± 0.25 °C Gradient Yes on all formats Max /min gradient Temp. 29 °C / 1 °C Number of recordable programmes 1000 Fan noise emission 50 dB 50 dB (1 block in FCT) 55 dB (2 blocks in FCT) 50 dB (only 1 block in FCT) 55-58 dB (4 blocks in FCT) Number of peltier/block elements 8 Heated/adjustable lid temperature Yes from 35 °C to 115 °C or inactive Pressure of the heating cover Adjustable Program interface 7" 1080 pHD 10" 1080 pHD Dimensions L x W x H(mm) 430 x 260 x 200 470 x 535 x 330 700 x 535 x 200 Weight (kg) 11.8 30 45 Voltage 110-230 V, 50-60 Hz Alpha Cycler 1 Alpha Cycler 4 Cat. No. Number of blocks € 789980 1 block 96-well N/A - 789981 1 block 384-well N/A - Cat. No. Number of blocks € 789982 4 block 96-well N/A - 789983 4 block 384-well N/A - 789984 1 x 96-well block and 3 x 384-well blocks N/A - 789985 2 x 96-well blocks and 2 x 384-well blocks N/A - 789986 3 x 96-well blocks and 1 x 384-well block N/A - Alpha Cycler 2 Cat. No. Number of blocks € 999610 2 block 96-well N/A - 999611 2 block 384-well N/A - 999612 1x 96-well block and 1 x 384-well block N/A - AlphaCycler Thermal Cyclers

Molecular Biology 148 Temperature range / Temperature accuracy at 55 °C 4 °C to 100 °C/ ‹ ±0.25 °C Block uniformity at 55 °C ‹±0.3 °C Gradient range 30 °C to 80 °C Maximum / minimum gradient 29 °C (19 °C for 384 plates) / 1 °C Temperature setting of the heated cover 100 °C to 115 °C or inactive Number of recordable programmes 1000 Dimensions (L x W x H) 350 x 210 x 180 mm Voltage 100-230 V, 50-60 Hz Cat. No. Description € 798917 Thermal Cycler 3Prime 24 x 0.2 ml N/A - 798918 Thermal Cycler 3Prime 18 x 0.5 ml N/A - 798919 Thermal Cycler 3PrimeX 48 x 0.2 ml N/A - 798920 Thermal Cycler 3PrimeX 30 x 0.5 ml N/A - 798921 Gradient thermal cycler 3PrimeG 48 x 0.2 ml N/A - 798922 Gradient thermal cycler 3PrimeG 30 x 0.5 ml N/A - Blocks - Block changes for 3Prime thermal cyclers should be carried out by a qualified engineer. Cat. No. Description € 798924 Block 3Prime 24 x 0.2 ml N/A - 798925 Block 3Prime 18 x 0.5 ml N/A - 798926 Block 3PrimeX 48 x 0.2 ml N/A - 798927 Block 3PrimeX 30 x 0.5 ml N/A - hHigh flexibility, easy programming, small footprint, pre-stored protocols hFast temperature rise up to 3 °C/sec hColour touch screen, data logging to PC via front USB port hCycle gradient available on all 3PrimeG devices or with a simple software update on 3PrimeX devices via USB stick h3PrimeG and 3PrimeX with 4-year warranty and 80 000 cycles or 4 years of block lif hInterchangeable blocks 24 and 48 x 0.2 ml and 18 and 30 x 0.5 ml hFunction to calculate the gradient hPossibility of updating the software to access the cycle gradient Cat.No. Description € 798923 3PrimeX UpGrade service to enable gradient mode N/A - Option Thermal cyclers 3Prime WEB Enter the Cat. No. on dulis.nl to display the Techne PRIME thermal cycler selection guide Thermal cyclers

Molecular Biology 149 All prices are indicative and exclude VAT. Visit www.dulis.nl Electrophoresis / Generators 022099 hAt constant voltage or constant current hSelection in 1 V or 1 mA increments hLED display h2 outputs hCompact and lightweight hTimer 1 to 999 minutes or continuous with alarm function hPolycarbonate case, aluminium base Power 60 W 10-400mA 10-300 V 120 W 10-400mA 10-500 V Operating temperature Ambient temperature at + 40 °C Ambient temperature at + 40 °C Dimensions (mm) 13x 189 x 85 140 x 191 x 84 Weight 0.9 kg NC Minuteur 1 to 999 minutes with alarm 1 to 999 minutes with alarm Cat. No. 022099 022237 € N/A - N/A - Mini Pro 300 V generator Mini 500 V generator 022237 Model Mini Pro 300 V and Mini 500 V generators hFor agarose or acrylamide gel electrophoresis hIntuitive programming hCan be positioned horizontally or vertically hTouch screen with automatic rotation hConstant voltage or constant current mode hOverload, change of load and no load detection hStackable unit hUltra-compact two-output generator hBasic device for constant voltage application h2 constant voltages to choose from, 100 or 200 V Constant voltage (V) 100 or 200 Max. current (mA) 200 Max. power (W) 40 Output Terminals 2 Power supply 230 - 240 V, 50 - 60 Hz Dim. W x D x H (cm) 16 x 11.5 x 5.7 Weight (g) 400 Cat.No. 062693 € N/A - Current (mA) 5 to 400 Current increment (mA) 5 Voltage (V) 10 to 300 Voltage increment (V) 5 Max power (W) 80 Timer 1 min to 99 h 59 min Output Terminals 4 Screen size (mm) 50.8 x 50.8 Power supply 230 V, 50 Hz Dim. (cm) W x D x H 21.5 x 15.5 x 9.75 Weight (kg) 1.6 Cat.No. 062694B € N/A - MyVolt mini Power supply MyVolt touch Power supply

Molecular Biology 150 Electrophoresis / Generators The Apelex range of generators covers all applications in electrophoresis. hFront panel with touch buttons hAutomatic restart in case of power failure hConstant parameter display via LED hTimer for all models except PS 304 hFor 4 mm diameter plug Max. Voltage. (V) 300 300 500 600 1000 1000 3000 Max. current. (mA) 400 500 300 800 600 600 300 Max power (W) 100 100 100 300 300 300 300 Output Terminals 4 4 4 3 3 3 2 Constant voltage and current x x x x x x x Constant voltage, current, power x x Timer x x x x x x Integrator x x Programmable x x Touch buttons x x x x x x x Applications DNA/RNA agarose electrophoresis x x x x o o o RFLP and DNA fragments x x x x o o o PCR Screening x x x x o o o Nucleic acid sequencing x Isoelectric focusing SSCP x x Protein electrophoresis o x x x x x o SDS PAGE preparatory gels o x x x x x o Semi-dry blotting o x x o o o Western Blotting x x o o Cat. No. 049197B 049190B 049189B 049191 049192 049195 049194 € N/A - N/A - N/A - N/A - N/A - N/A - N/A - X: recommended o: optional Features PS 304 XL PS 305 XL PS 503 XL PS 608 PS 1006 PS 1006 P PS 9009 TX 2 programmable models PS 1006 P and PS 9009 TX Model Model EV2310 EV2650 EV2320 EV3020 EV3610 EV3150 EV3330 EV3620 Max. voltage (V) 300 600 3000 300 600 1200 3000 6000 Max. current (mA) 1000 500 150 2000 1000 500 300 150 Max. power (W) 150 150 150 300 300 300 300 300 Output Terminals 4 Timer 0 to 99 hrs 59 min For plug diameter (mm) 4 4 4 4 4 4 4 2 Applications DNA sequencing x x Flat bed isoelectric focusing x x x Horizontal Gel x x x x x x x Long vertical gel x Vertical gel x x x x x x x Electroelution x x x x Western blotting x Semi-dry blotting x Mini-western blotting x Semi-dry mini blotting x Cat. No. 324083 324084 324085 324086 324087 324088 324089 324093 € N/A - N/A - N/A - N/A - N/A - N/A - N/A - N/A - hLCD display with user assistance in case of error hAutomatic restart in case of power failure h9 different programmes with up to 9 steps each hReal-time clock with recording of operating parameters and retrieval via USB interface X: recommended Consort Electrophoresis generators LCD display Apelex electrophoresis generators Cat. No. Description € 324068 Reducer with 4 mm diameter banana plugs for 2 mm outlet N/A - 324069 2 mm banana plug adapter retractable socket for 4 mm outlets N/A - Accessories

Molecular Biology 151 All prices are indicative and exclude VAT. Visit www.dulis.nl An economical and ready-to-use solution: choose the RunOne + Multicaster chamber kit (the Cat. No. appear in the Multicaster table) Cat. No. 038420 Mini agarose gel 5.4 x 5.9 N/A - 038421 Acrylamide gel (landscape) with cover 11 x 6 N/A - 038422 Mini acrylamide gel with cover 5.7 x 5.9 N/A - Tray for Dim. W x H (cm) € 4 units Cat. No. 038423 Portrait 6 x 9.5 cm 11.5 x 9.5 N/A - 035425 Medium 11.5 x 8.9 cm 11.5 x 9.5 N/A - 035426 Long 11.5 x 11.9 cm 11.5 x 11.5 N/A - For agarose gel size Dim. W x H (cm) € the tray Cat. No. 039030 Casting system N/A - Description € Cat. No. 038401 Complete RunOne system N/A - Description2 € Accessories for the RunOne tank UV-transparent tray (different sizes depending on the application). Specially designed to facilitate loading into the RunOne tank (red, placed under the gel and allows for a contrast between the agarose and the wells). Red tray for visualization of deposition wells Pouring system For horizontal electrophoresis in mini-gels. All in one: • electrophoresis tanks with 6-compartment tray; • generator with integrated timer; • accepts 2 types of agarose and polyacrylamide gels simultaneously. Optional accessories: Multicaster system, pre-cast gels. The system with timer comes complete with generator and 220V transformer, a pouring tray with 6 compartments, 6 trays for agarose gels (4 minigels, 2 maxigels) with lid and 2 combs. Trays for polyacrylamide gels (4 minigels, 2 maxigels) are ordered separately. hThe tray has 6 moulded compartments, into which 1 to 6 gels can be poured hGel sizes: minigel (5.2 x 5.9 cm) and standard (10.7 x 5.9 cm) hCapacity: 4 minigels and 2 standard gels hWatertight, without joints or adhesives hGenerator with integrated timer hRemovable generator with 3 voltage selections: 25 V, 50 V or 100 V hBuffer volume required: 350 ml for 2 minigels or 1 standard gel hMaximum number of samples: 17 to 96 depending on the tray 038420 038422 038423 035425 035426 039030 038421 Horizontal electrophoresis RunOne electrophoresis system for nucleic acids

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