Macherey-Nagel -

NucleoSpin DNA extraction kit for tissues and organs MACHEREY-NAGEL

• Extraction of DNA from tissues or organs
• Extraction on silica membrane column by centrifugation
• For fragment sizes from 200 bp to 50 kbp
• Output from 1 to 30 µl

NucleoBond PC EF endotoxin-free plasmid extraction kit MACHEREY-NAGEL

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• Extraction of endotoxin-free plasmid DNA from bacterial culture
• Gravity anion exchange chromatography extraction
• For starting sample volume of 30 ml to 2 l depending on the kit used

Plasmid extraction kit for NucleoBond PC transfection MACHEREY-NAGEL

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• Extraction of plasmid DNA for transfection from bacterial culture
• Extraction by gravity anion exchange chromatography
• For starting sample volume of 1 ml to 2 l depending on the kit used

MACHEREY-NAGEL NucleoSpin transfection plasmid extraction kit

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• Extraction of plasmid DNA for transfection from bacterial culture
• Extraction on silica membrane column by centrifugation
• For starting sample volume of 1 to 5 ml

NucleoSpin and NucleoSpin EasyPure plasmid extraction kit MACHEREY-NAGEL

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• Rapid extraction of plasmid DNA from bacterial culture
• Extraction on silica membrane column by centrifugation
• Ready-to-use DNA for restriction fragment analysis, cloning, PCR, sequencing and cell transfection
• NucleoSpin Plasmid EasyPure: very fast procedure with a single step of combined washing and drying
• For starting sample volume of 1 to 10 ml depending on the number of copies of the plasmid

NucleoSpin blood DNA extraction kit MACHEREY-NAGEL

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• DNA extraction from blood, plasma or serum
• Extraction on silica membrane column by centrifugation
• Sample quantity:
- 5 to 200 µl for Mini kit
- 0.2 to 2 ml for Midi kit
- 2 to 10 ml for Maxi size kit
• For fragment sizes from 50 bp to 50 kpb
• Performance:
- 4 to 6 µg for Mini kit
- 40 to 60 µg for Midi kit
- 200 to 300 µg for Maxi kit

DNA extraction kit from bacterial culture NucleoMag MACHEREY-NAGEL

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• Kit for DNA purification from bacterial and yeast cultures
• Magnetic bead extraction
• Kits compatible with automatic purification systems
• For sample quantity < 40 mg of bacteria (108 to 109 bacteria) or < 30 mg of yeast
• Yield from 5 to 25 µg

Purification of PCR products and fragments from NucleoSpin MACHEREY-NAGEL gel strips

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• Purification of DNA or RNA from agarose gels or polyacrylamide gels and purification of PCR products
• Silica membrane column purification by centrifugation
• For sample:
- < 400 µl PCR reaction mix, < 400 µg agarose gel for Mini kit
- < 4 ml of PCR reaction mixture , < 4 mg of agarose gel for Midi format kit
- < 10 ml of PCR reaction mixture , < 10 mg of agarose gel for Maxi format kit
• For fragment sizes from 50 bp to 20 kbp
• Elution volume:
- from 15 to 30 µl for the Mini kit
- from 200 to 400 µl for the Midi format kit
- 1 ml for the Maxi size kit

Macherey-Nagel NucleoMag PCR Product Purification Kit

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• PCR product purification kit
• Magnetic bead purification
• Kits compatible with automatic purification systems
• For sample quantity < 50 µl
• For fragment sizes from 150 bp to 10 kpb
• Elution volume from 25 to 100 µl

NucleoMag NGS clean-up resin MACHEREY-NAGEL

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• Nucleic acid purification and size selection kit for NGS library construction
• Magnetic bead purification
• Kits compatible with automatic purification systems
• For sample quantities of 50 to 150 µl
• For fragment sizes from 150 to 800 bp
• Elution volume of 10 to 100 µl

Protino Ni-NTA protein purification kit MACHEREY-NAGEL

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• Purification of poly-histidine-tagged proteins by affinity chromatography
• For all types of proteins (small proteins, large protein complexes, proteins with low expression levels)
• Purification under native or denaturing conditions
• Matrix: 6% agarose suspension (cross-linked), Ni2+ loaded