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Molecular biology Transfection of nucleic acids Reagents for nucleic acid transfection

Reagents for nucleic acid transfection

Magnetofection

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Magnetofection combines chemical and physical approaches. This involves combining nucleic acids, transfection reagents (polymers, lipids) or viruses with magnetic nanoparticles to improve transport of the material to the cell, directed by a specific magnetic field.

Lipofection

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The most commonly used non-viral approach. Effective, suitable for all types of nucleic acid and a wide range of cells.

Polyfection

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• High transfection efficiency and transgenic expression with small amounts of DNA
• High production of intracellular proteins while preserving viability
• High production of secreted proteins while minimising cellular stress
• Compatible with all culture media
• Biodegradable

Application :
• DNA transfection in immortal cell lines : HEK-293, NIH-3T3, CHO, COS, HeLa, ..