Molecular Biology - Nucleic Acids Extraction et purification d'acides nucléiques sur colonne Extraction et purification d'ADN plasmidique

Extraction et purification d'ADN plasmidique

NucleoBond PC EF endotoxin-free plasmid extraction kit MACHEREY-NAGEL

Ontdekken

• Extraction of endotoxin-free plasmid DNA from bacterial culture
• Gravity anion exchange chromatography extraction
• For starting sample volume of 30 ml to 2 l depending on the kit used

Plasmid extraction kit for NucleoBond PC transfection MACHEREY-NAGEL

Ontdekken

• Extraction of plasmid DNA for transfection from bacterial culture
• Extraction by gravity anion exchange chromatography
• For starting sample volume of 1 ml to 2 l depending on the kit used

MACHEREY-NAGEL NucleoSpin transfection plasmid extraction kit

Ontdekken

• Extraction of plasmid DNA for transfection from bacterial culture
• Extraction on silica membrane column by centrifugation
• For starting sample volume of 1 to 5 ml

NucleoSpin and NucleoSpin EasyPure plasmid extraction kit MACHEREY-NAGEL

Ontdekken

• Rapid extraction of plasmid DNA from bacterial culture
• Extraction on silica membrane column by centrifugation
• Ready-to-use DNA for restriction fragment analysis, cloning, PCR, sequencing and cell transfection
• NucleoSpin Plasmid EasyPure: very fast procedure with a single step of combined washing and drying
• For starting sample volume of 1 to 10 ml depending on the number of copies of the plasmid

illustra™ plasmidPrep Cytiva

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GeneMATRIX DNA purification kit for routine applications

Ontdekken

• GeneMATRIX is a new generation resin that selectively binds nucleic acids to silica composite structures
• For the most common linear and circular DNA purification applications:
- Purification of DNA from a PCR reaction while removing primers, nucleotides, short double-stranded DNA fragments (' 20 bp), polymerases...
- Purification of DNA up to 10 kpb after migration in an agarose gel (TAE or TBE) while eliminating dyes such as BET, EDTA, enzyme inhibitors..
- Purification of plasmid from a bacterial culture while removing RNA, single-stranded DNA, enzymes, endotoxins, lipids..