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Filtration Proteins Sample preparation

Sample preparation

Cytiva Desalting Columns

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Applications:
• Buffer exchange, desalting
• Clean-up of proteins, peptides, sugars, and more biomolecule
• Removal of small contaminants (salts, dyes, radioactive labels) from samples using gravity flow

Protocol:
• Gel filtration with separation according to molecular weight
• Sephadex™ G-25 media, exclusion limit: > 5000 Mr
• Sephadex™ G-10 media, exclusion limit: > 700 Mr