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Cell culture - Serums - Media Sample preparation Blood sample preparation

Blood sample preparation

Leucosep and Blood Sep filter

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• Simple and efficient separation of PBMCs (lymphocytes and mononuclear cells) from blood or bone marrow
• Concentration directly from whole blood
• Rapid separation in 15 minutes at room temperature
• During centrifugation, lymphocytes and mononuclear cells are separated from unwanted elements by density gradient and concentrate in a phase above the separating medium
• When separation is complete, at the time of harvest, the porous barrier minimizes contamination of the enriched fraction with red blood cells
• Pre-filled with LeucosepTM separation medium (density 1.077 g/ml)
• Available unfilled for use with all other separation media on the market

Lymphocyte separation media

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Lymphocyte Separation Medium (LSM) is a ready to use, sterile based on Ficoll, a polymer with a high molecular weight (400.000) and a perfectly defined density of 1.077 +/- 0.001. The LSM is intended for the preparation of lymphocyte solutions from whole blood. The LSM should be kept at 4 °C and away from light. A certificate of quality can be made available on request.

System OncoQuick Greiner Bio-One

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Enrichment of circulating tumour cells released from whole blood.

OncoQuick® is a simple to use, rapid and efficient system for the enrichment of circulating tumour cells released by solid tumours or malignant melanomas. OncoQuick® comes as a polypropylene tube with a porous barrier inserted above a specially developed separation solution. Up to 30 mI of anticoagulated blood is put directly into the OncoQuick® tube and centrifuged. Apart from erythrocytes and granulocytes, the separation medium virtually eliminates lymphocytes and mononuclear cells. The circulating tumour cells are concentrated at the interface. Once collected, the fraction enriched with cells is washed.

The tumour cells are available for all classic diagnostic methods. OncoQuick® was developed in cooperation with Hexal Gentech and Greiner Bio-One and is reserved for research use only!

Nylon wool fibre

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• For separating T-cells from B-cells
• Instructions on request

Density gradient Ficoll and Percoll

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Blood Sep Filter

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Filter held by two rings moulded into the tube. These ensure that the filter remains in place during centrifugation.

Leucosep "ready to use" tubes

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• Reduces the risk of contamination
• Time saving
• Stable 36 months at 2-20 °C
• Store in a dark place