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Blood sample preparation

Leucosep and Blood Sep filter

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• Simple and efficient separation of PBMCs (lymphocytes and mononuclear cells) from blood or bone marrow
• Concentration directly from whole blood
• Rapid separation in 15 minutes at room temperature
• During centrifugation, lymphocytes and mononuclear cells are separated from unwanted elements by density gradient and concentrate in a phase above the separating medium
• When separation is complete, at the time of harvest, the porous barrier minimizes contamination of the enriched fraction with red blood cells
• Pre-filled with LeucosepTM separation medium (density 1.077 g/ml)
• Available unfilled for use with all other separation media on the market

Lymphocyte separation media

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Lymphocyte Separation Medium (LSM) is a ready to use, sterile based on Ficoll, a polymer with a high molecular weight (400.000) and a perfectly defined density of 1.077 +/- 0.001. The LSM is intended for the preparation of lymphocyte solutions from whole blood. The LSM should be kept at 4 °C and away from light. A certificate of quality can be made available on request.

Nylon wool fibre

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• For separating T-cells from B-cells
• Instructions on request

Blood Sep Filter

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Filter held by two rings moulded into the tube. These ensure that the filter remains in place during centrifugation.

Leucosep "ready to use" tubes

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• Reduces the risk of contamination
• Time saving
• Stable 36 months at 2-20 °C
• Store in a dark place