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The buffer TAE (Tris-Acetate-EDTA) is used in agarose gel electrophoresis for applications requiring high resolution and separation under a low voltage for high molecular weight double stranded DNA. The solutions are aseptically microfiltered.
• Dissolve in pure water to the indicated final concentration • Makes 10L of 1 X solution: 1 x contains: 89 mm Tris, 89 mm borate, 2 mm EDTA, pH 8.2 -8.4 li >
• With coloured cap • Sterile, single pack • Manufactured in a clean room • In medical grade polycarbonate • Electrode treatment with 11 washing steps to ensure homogeneous pulses
• For DNA samples to be loaded onto acrylamide or agarose gels • Composition: • 100 mM tris-HCL, pH 8.0 • Xylene Cyanol • Bromophenol Blue • Glycerol, 30 • 100mM EDTA, pH 8.0
• For RNA on polyacrylamide or agarose gel • Allows you to optimise the migration according to the size of the expected fragment(s) • Composition: Tris-HCL pH 7.6 (10 mM), EDTA pH 8 (60 mM), Xylene cyanol (0.03%), Bromophenol Blue (0.03%), Orange G (0.15%), glycerol (60%)