By affinity - Laboratoriumuitrusting

HisTrap™ columns: purification of histidine-tagged proteins

HisTrap HP (High Performance): for purification of histidine-tagged protein after sample filtration. Particle size: 34 µm.

HisTrap FF Crude: for purification of histidine-tagged proteins. No need for filtration of the samples.

HisTrap FF (Fast Flow): for purification of histidine-tagged recombinant proteins from large volume of filtered sample. Particle size: 90 µm.

TALON® Superflow™ and HiTrap™ TALON® Crude

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TALON®, is a new cobalt-based immobilized metal affinity chromatography medium (IMAC) offering enhanced selectivity for histidine-tagged proteins

Suitable for purification of histidine-tagged proteins when Ni²⁺is not the optimal choice of metal ion.

HisTrap™ EXCEL® columns

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HisTrap EXCEL columns are prepacked with Ni Sepharose excel affinity media for capture and purification of histidine-tagged proteins secreted into eukaryotic cells.

HiTrap™ Chelating and HiTrap IMAC™ columns

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For optimizing purification of histidine-tagged proteins using different kinds of metal ions as Cu²⁺, Zn²⁺, Ni²⁺, Co²⁺, Fe³⁺.

GSTrap™ columns

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StrepTrap™ and MBPTrap™ columns

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HiTrap™ columns

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• HiTrap columns are available in 1 ml and 5 ml bed volume
• Each HiTrap™ column is equiped with one 1/16" male luer and one 1/16" female luer. It is possible to connect columns to each other for achieving columns of variable volume from 1 to 20 ml as required
• Concentration or desalting samples
• Purification of recombinant proteins
• Purification of immunoglobulins

Acronyms and abbreviations: Q = Quaternary ammonium / DEAE = Diethylaminoethyle / CM = Carboxymethyle / S = Methylsulfonate / ANX = Diethylaminopropyle

HiTrap Capto™: designed to meet industrial requirements for the production of biological molecules.

HiTrap NHS: high-performance medium designed for easy coupling of ligands containing primary amines.

HiTrap Protein A and HiTrap Protein G: designed for preparative purification of monoclonal and polyclonal antibodies from ascites, serum, and cell culture supernatants.

HiTrap MabSelect™, MabSelect SuRe™ and MabSelect Xtra™: developed to meet the productivity and sanitisation requirements of industry to develop large-scale antibody purification procedures.

HiTrap IgM: designed for purification of monoclonal IgM.

HiTrap IgY: designed for purification of IgY.

HiTrap IXSelect: pour la purification du facteur de coagulation IX.

IEX HiTrap™ Capto Selection kit

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Kit containing 5 HiTrap™ Capto columns, coupled to different loaded ligands:
• HiTrap™ Capto Q: strong anion exchanger
• HiTrap™ Capto DEAE: weak anion exchanger
• HiTrap™ Capto S: strong cation exchanger
• HiTrap™ CaptommC: multimodal cation exchanger, works at high and low ionic strength
• HiTrap™ Capto Adhere: designed for A post-protein purification of monoclonal antibodies

Capto L medium

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Chromatography medium for the capture of antibodies and antibody fragments.

MabSelect matrix

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Chromatography media for monoclonal antibodies purification in large scale.

Protein G sepharose 4 FF

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• Recombinant protein G coupled to Sepharose 4 Fast Flow (4 % agarose matrix)
• Application: purification of monoclonal or polyclonal IgG antibodies
• No specific albumin binding
• Dynamic capacity biding: 18 mg human IgG / ml medium
• pH working range: 3 to 9
• Storage: 2 to 8 °C

nProtein A Sepharose 4 Fast Flow

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• nProtein A coupled to Sepharose 4 Fast Flow (4% agarose matrix)
• Application: purification of monoclonal and polyclonal antibodies
• Highly stable with minimal nonspecific adsorption
• Binding capacity: 35 mg human IgG / ml of matrix
• pH range: 3 - 9
• storage: 4 to 8 ° C