Cell culture - Serums - Media Greiner Bio-One 3D cell culturing - Magnetic suspensions and bioprinting 3D cell culture - Spheroids - Organoids: M3D Greiner Bio-One Kit

3D cell culture - Spheroids - Organoids: M3D Greiner Bio-One Kit

System for 3D cell culture thanks to the transient magnetization of cells that naturally attach to the NanoShuttle-PL magnetic beads.
Thanks to the magnetic force, the cells are ready for either magnetic levitation or spheroid printing, to form 3D in vitro representation models.

• in vitro reproduction of the native tissue environment
• Versatile system compatible with all cell types even in co-culture
• 3D model formation in a few hours after incubation with magnetic beads
• Allows cell co-culture
• No impact of the magnetized beads on the cells (proliferation, viability, phenotype ...)
• Centered positioning of the spheroids
• No specialized equipment, no media, no artificial substrate
• Easy to handle, no sample loss

Selection guide - Greiner Bio-One M3D cell culture system

Application note - Interest of M3D in automated 3D culture systems

Note d'application Multi-Magpen Greiner Bio-One - transfert magnétique des cultures cellulaires 3D Access to Greiner Bio-One publications

Source: Greiner Bio-One

Varianten en accessoires

Magnetic levitation

Spheroid printing

Magnetic ring printing and imaging system

Magnetic NanoShuttle-PL

Accessory

Multi-MagPen 24 and 96 wells

Accessories

Transiently and non-invasively magnetized cells are placed in a Cell-Repellent plate. A magnet placed above the plate attracts the cells to the air/medium interface. Magnetic forces bundle the cells together, favouring intercellular interactions at the origin of the extracellular matrix synthesis.

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6-well Bio-Assembler kit (magnetic levitation), includes: 2 x 600 µl vials of NanoShuttle-PL, 1 x 6-well levitating drive, 1 x 6-well concentrating drive, 2 x 6-well cell culture multiwell plates with Cell-Repellent surface

657840 - 6-well Bio-Assembler kit (magnetic levitation), includes: 2 x 600 µl vials of NanoShuttle-PL, 1 x 6-well levitating drive, 1 x 6-well concentrating drive, 2 x 6-well cell culture multiwell plates with Cell-Repellent surface

Verkoopeenheid : 1

24-well Bio-Assembler kit (magnetic levitation), includes: 2 x 600 µl vials of NanoShuttle-PL, 1 x 24-well levitating drive, 1 x 24-well concentrating drive, 2 x 24-well cell culture multiwell plates with Cell-Repellent surface, 1 Lid

662840 - 24-well Bio-Assembler kit (magnetic levitation), includes: 2 x 600 µl vials of NanoShuttle-PL, 1 x 24-well levitating drive, 1 x 24-well concentrating drive, 2 x 24-well cell culture multiwell plates with Cell-Repellent surface, 1 Lid

Verkoopeenheid : 1

Transiently and non-invasively magnetized cells are placed in a plate and a magnetic holder will be positioned underneath that plate, keeping the cells at the bottom of the well. In a few minutes or even hours, the plate containing the spheroids can be removed from the magnetic holder and cultured over the long term.

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96-well Bioprinting kit (spheroid bioprinting), includes: 3 x 600 µl vials of NanoShuttle-PL, 1 x 96-well spheroid drive, 1 x 96-well holding drive, 2 x 96-well cell culture microplates (black, with µClear® film bottom) with Cell-Repellent surface

655841 - 96-well Bioprinting kit (spheroid bioprinting), includes: 3 x 600 µl vials of NanoShuttle-PL, 1 x 96-well spheroid drive, 1 x 96-well holding drive, 2 x 96-well cell culture microplates (black, with µClear® film bottom) with Cell-Repellent surface

Verkoopeenheid : 1

655830 - M3D CELL CULTURE MEDIUM, 96 WELLS, SPHEROIDE DRIVE AND HOLDING DRIVE

Verkoopeenheid : 1

655840 - 96-well Bioprinting kit (spheroid bioprinting), includes: 3 x 600 µl vials of NanoShuttle-PL, 1 x 96-well spheroid drive, 1 x 96-well holding drive, 2 x 96-well cell culture microplates (clear) with Cell-Repellent surface

Verkoopeenheid : 1

384-well Bioprinting kit (spheroid bioprinting), includes: 2 x 600 µl vials of NanoShuttle-PL, 1 x 384 spheroid drive, 1 x 384-well holding drive, 2 x 384-well cell culture microplates (black, µClear® film bottom) with Cell-Repellent surface

781841 - 384-well Bioprinting kit (spheroid bioprinting), includes: 2 x 600 µl vials of NanoShuttle-PL, 1 x 384 spheroid drive, 1 x 384-well holding drive, 2 x 384-well cell culture microplates (black, µClear® film bottom) with Cell-Repellent surface

Verkoopeenheid : 1

073054 - Bioprinting kit for 3D culture and spheroid formation - 384-well

Verkoopeenheid : 1

Based on the 3D magnetic Spheroid printing, transiently and non-invasively magnetized cells thanks to Nanoshuttle-PL beads are positioned in a ring shape mimicking certain in-vivo patterns and their functions (ex. contraction).

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96-well Bio Assay kit: 3 x 600 µl vials of NanoShuttle, 1 x 6-well levitating drive, 1 x 96-well spheroid drive, 1 x 96-well holding drive, 1 x 96-well ring Drive, 1 x 96-well Deepwell plate, 2 x 6-well cell culture multiwell plates with cell-repellent surface (655970), 2 x 96-well cell culture microplates with cell-repellent surface (657970)

655846 - 96-well Bio Assay kit: 3 x 600 µl vials of NanoShuttle, 1 x 6-well levitating drive, 1 x 96-well spheroid drive, 1 x 96-well holding drive, 1 x 96-well ring Drive, 1 x 96-well Deepwell plate, 2 x 6-well cell culture multiwell plates with cell-repellent surface (655970), 2 x 96-well cell culture microplates with cell-repellent surface (657970)

Verkoopeenheid : 1

384-well Bio Assay Kit: 2 Nanoshuttle flask 600 µl / For plate 384-well: 1 spheroid drive ; 1 holding drive ; 2 cell repellent plates (781970) / For 6-well plates: 1 levitating drive ; 2 cell repellent plates (657970)

781846 - 384-well Bio Assay Kit: 2 Nanoshuttle flask 600 µl / For plate 384-well: 1 spheroid drive ; 1 holding drive ; 2 cell repellent plates (781970) / For 6-well plates: 1 levitating drive ; 2 cell repellent plates (657970)

Verkoopeenheid : 1

• Nano-particles assembly (50 nm diameter), does not bind any specific receptors, works with all cell types - attaches to the plasma membrane electrostatically
• Consists of biocompatible gold, iron oxide and Poly-L-Lysine
• No effects on proliferation, viability, metablism, oxydative stress, phenotype
• Does not disturb experimental techniques such as immunohistological labelling, fluorescence detection, HR microscopy, Western Blot, PCR/qPCR ...

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657843 - NanoShuttle 600 µl magnetic beads Tube NanoShuttle, 3-unit pack

Verkoopeenheid : 3

657846 - NanoShuttle 600 µl magnetic beads Tube NanoShuttle, 6-unit pack

Verkoopeenheid : 6

Magnetic tool for the individual manipulation of formed spheroids

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• Fast, easy and simultaneous transfer of multiple 3D cell cultures without pipetting
• Complete change of media by a simple pick-up-and-drop step
• Simplified co-culture of different cell types
• Ideal for facilitating immunohistochemical staining, blocking and washing of spheroids

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657825 - Sterile intermediate cover for M3D 6-well Bio-Assembler kit, 2 pieces per bag

Verkoopeenheid : 10

662825 - Sterile intermediate cover for M3D 24-well Bio-Assembler kit, 2 pieces per bag

Verkoopeenheid : 10

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