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Collagen 1 is present in numerous tissues and organs. When used as a fine coating on a culture surface, it enables the development, proliferation and differentiation of a large number of cell types.
Poly-D-Lysine is a synthetic molecule used to increase the attachment of cells to plastic and glass. It is a substitute that will facilitate the development, proliferation and differentiation of nerve cells and other cell types.
Matrigel is a solubilised basement membrane preparation extracted from the EHS tumour (Engelbreth-Holm-Swarm) which solidifies to form a structure equivalent to a basement membrane in terms of its composition, structure and physical properties.
The most important components of Matrigel are laminin, collagen IV, entactin and heparan sulphate proteoglycans.
Matrigel is strongly recommended for epithelial cell cultures. It is effective for the differentiation of numerous cell types such as hepatocytes and mammary epithelial cells, and results in the formation and organisation of endothelial cells in capillary tubes and the regeneration of neurites.
The Matrigel coating causes the polarisation of single layer epithelial cells, and the proliferation and migration of smooth muscle cells.
It plays a role in regulating cell growth, differentiation and attachment. It promotes the culture of a large number of cell types such as macrophages, neurons, smooth muscle cells, Sertoli cells, epithelial cells in the trachea, keratinocytes, hepatocytes.
Laminin is a major component of the basement membrane. This 900 kDa glycoprotein is composed of three sub-units. Laminin attaches to components of the basement membrane (collagen IV, heparan sulphate proteoglycan) or to receptors on cell surfaces (integrin). Laminin causes neurite growth, has an effect on migration, growth, morphology and the attachment of Schwann cells and olfactory neurons, and is involved in tissue repair. It also regulates the growth of a large number of cell types including macrophages, hepatocytes, keratinocytes, melanocytes, myocardiocytes, cells transformed by Ras, haematopoietic stem cells. Furthermore, laminin has an effect on human neutrophils (oxydating mechanisms), suppresses lymphocyte responses to T cell mitogens, plays a role in polarising epithelial cells and can be used to study tumourous invasion.
Fibronectin is a 440-450 kDa glycoprotein composed of 2 sub-units of 220-250 kDa linked by a pair of C-terminal disulphide bonds. Fibronectin is present in plasma as a protein dimer (soluble) and in the extracellular matrix on cell surfaces as a polymer (heavy weight molecule).
The main function of fibronectin is to attach the cells to the extracellular matrix.
Fibronectin can be used as a culture supplement in a serum-free medium or as a fine coating. Coated fibronectin brings about the adhesion and proliferation of BHK and CHO cells, endothelial cells, fibroblasts, smooth muscle cells, neurons, bronchial epithelial cells.
Chemically-defined, animal-free cell culture surfaces designed to enhance cell performance. They enhance the post-thaw attachment, proliferation, and recovery of cells, and promote the differentiation of numerous cell types known to have poor attachment properties in serum-reduced or serum-free cultures (e.g. primary cultures, transfected cells...).